Detailed information of TA system TA155424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 2790022..2790683
• Replicon: chromosome
• Accession: NZ_CP052068
• Organism: Ralstonia solanacearum strain FJAT91.F50

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A9E7Z845
• Locus tag: HI824_RS12905
• Protein ID: WP_016727753.1
• Coordinates: 2790270..2790683 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A9E7Z6P3
• Locus tag: HI824_RS12900
• Protein ID: WP_019717749.1
• Coordinates: 2790022..2790273 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI824_RS12870	2785516..2785665	+	150	WP_155738951.1	hypothetical protein	-
HI824_RS12875	2785726..2786340	-	615	WP_157698853.1	hypothetical protein	-
HI824_RS12880	2787041..2787757	+	717	WP_049800424.1	Crp/Fnr family transcriptional regulator	-
HI824_RS12885	2788432..2788647	-	216	WP_071624182.1	glycine zipper 2TM domain-containing protein	-
HI824_RS12890	2789044..2789259	+	216	WP_071615547.1	hypothetical protein	-
HI824_RS12895	2789413..2789645	-	233	Protein_2518	hypothetical protein	-
HI824_RS12900	2790022..2790273	+	252	WP_019717749.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HI824_RS12905	2790270..2790683	+	414	WP_016727753.1	type II toxin-antitoxin system VapC family toxin	Toxin
HI824_RS12910	2790755..2791777	-	1023	WP_086706232.1	DNA cytosine methyltransferase	-
HI824_RS12915	2791917..2792210	+	294	WP_020371973.1	hypothetical protein	-
HI824_RS12920	2792598..2793443	+	846	WP_157698852.1	hypothetical protein	-
HI824_RS12925	2793677..2794321	-	645	WP_086706230.1	hypothetical protein	-
HI824_RS12930	2794397..2794549	-	153	WP_157698851.1	hypothetical protein	-
HI824_RS12935	2794558..2795121	-	564	Protein_2526	hypothetical protein	-
HI824_RS12940	2795074..2795361	+	288	WP_086706365.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2784554..2785519	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-67)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15586.89 Da
• Isoelectric Point: 6.2337

>T155424 WP_016727753.1 NZ_CP052068:2790270-2790683 [Ralstonia solanacearum]
MSYLIDTNVLSELRRKAPDARVVAWMQDRPRQSLYLSVLTLGEIRKGIERLDDAARRQHLIDWLEVELPNYFLGRLLDID
AHTADRWGRLMSSAGRPLPAIDGLLAATALQHDLTLVTRNTKDFAGLDVQLINPWEA

Nucleotide

• Length: 414 bp

>T155424 NZ_CP068801:c3168140-3167766 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9305.49 Da
• Isoelectric Point: 5.0774

>AT155424 WP_019717749.1 NZ_CP052068:2790022-2790273 [Ralstonia solanacearum]
MQSWQMQAAKARFSDVVKRAADDGPQEITVHGRPVAVVISRALFDRLSGGGESLVSFMRQSPLADQDDVVFERERSLPRE
VEF

Nucleotide

• Length: 252 bp

>AT155424 NZ_CP068801:c3168561-3168187 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGTGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA