Detailed information of TA system TA155423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2115243..2116838
• Replicon: chromosome
• Accession: NZ_CP052068
• Organism: Ralstonia solanacearum strain FJAT91.F50

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HI824_RS09730
• Protein ID: WP_086706272.1
• Coordinates: 2115501..2116838 (+)
• Length: 446 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0S4UDK3
• Locus tag: HI824_RS09725
• Protein ID: WP_016725279.1
• Coordinates: 2115243..2115497 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI824_RS09700	2111306..2112232	-	927	WP_016727232.1	muramoyltetrapeptide carboxypeptidase	-
HI824_RS09705	2112229..2112780	-	552	WP_016727233.1	tRNA adenosine(34) deaminase TadA	-
HI824_RS09710	2112800..2113276	-	477	WP_011001399.1	6-carboxytetrahydropterin synthase QueD	-
HI824_RS09715	2113344..2113982	-	639	WP_020831956.1	7-carboxy-7-deazaguanine synthase	-
HI824_RS09720	2114300..2115139	+	840	WP_011001397.1	oxaloacetate decarboxylase	-
HI824_RS09725	2115243..2115497	+	255	WP_016725279.1	helix-turn-helix transcriptional regulator	Antitoxin
HI824_RS09730	2115501..2116838	+	1338	WP_086706272.1	type II toxin-antitoxin system HipA family toxin	Toxin
HI824_RS09735	2116835..2118037	+	1203	WP_023470034.1	MFS transporter	-
HI824_RS09740	2118263..2118991	+	729	WP_021155798.1	DsbA family protein	-
HI824_RS09745	2119004..2119567	-	564	WP_080624831.1	RNA 2',3'-cyclic phosphodiesterase	-
HI824_RS09750	2119748..2120446	-	699	WP_016725453.1	protocatechuate 3,4-dioxygenase subunit alpha	-
HI824_RS09755	2120443..2121183	-	741	WP_011001390.1	protocatechuate 3,4-dioxygenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-107)

Antitoxin

• Transcription regulator, HipB-like (10-66)

Sequences

Toxin

Protein

• Length: 446 a.a.
• Molecular weight: 48754.78 Da
• Isoelectric Point: 6.7232

>T155423 WP_086706272.1 NZ_CP052068:2115501-2116838 [Ralstonia solanacearum]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDGWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGTSKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVE
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Nucleotide

• Length: 1338 bp

>T155423 NZ_CP068801:3119865-3120359 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 8754.07 Da
• Isoelectric Point: 10.4693

>AT155423 WP_016725279.1 NZ_CP052068:2115243-2115497 [Ralstonia solanacearum]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAGTPSAA
PADW

Nucleotide

• Length: 255 bp

>AT155423 NZ_CP068801:3119575-3119868 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0S4UDK3