Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2699847..2700498 Replicon chromosome
Accession NZ_CP052065
Organism Lacticaseibacillus paracasei strain 347-16

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag GJS60_RS13365 Protein ID WP_003567661.1
Coordinates 2699847..2700230 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag GJS60_RS13370 Protein ID WP_003567665.1
Coordinates 2700250..2700498 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJS60_RS13360 2699142..2699663 - 522 WP_003567657.1 QueT transporter family protein -
GJS60_RS13365 2699847..2700230 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GJS60_RS13370 2700250..2700498 - 249 WP_003567665.1 antitoxin Antitoxin
GJS60_RS13375 2700566..2701702 - 1137 WP_003581082.1 alanine racemase -
GJS60_RS13380 2701689..2702063 - 375 WP_003567669.1 holo-ACP synthase -
GJS60_RS13385 2702232..2703740 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
GJS60_RS13390 2703866..2704009 - 144 WP_016380391.1 hypothetical protein -
GJS60_RS13395 2704040..2705428 - 1389 WP_003581096.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T155421 WP_003567661.1 NZ_CP052065:c2700230-2699847 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T155421 NZ_CP068801:2943798-2943905 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACTTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT155421 WP_003567665.1 NZ_CP052065:c2700498-2700250 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT155421 NZ_CP068801:c2943750-2943684 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References