Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 94562..95298 Replicon plasmid pKPN41953
Accession NZ_CP052037
Organism Klebsiella pneumoniae strain KPN41053

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag HH204_RS26420 Protein ID WP_004098919.1
Coordinates 94816..95298 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag HH204_RS26415 Protein ID WP_004213599.1
Coordinates 94562..94828 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HH204_RS26390 89794..90351 + 558 WP_004213590.1 recombinase family protein -
HH204_RS26395 90354..93323 + 2970 WP_004213592.1 Tn3 family transposase -
HH204_RS26400 93365..93859 + 495 WP_004213594.1 hypothetical protein -
HH204_RS26405 93920..94123 + 204 WP_004213596.1 hemolysin expression modulator Hha -
HH204_RS26410 94137..94367 + 231 WP_004213598.1 hypothetical protein -
HH204_RS26415 94562..94828 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
HH204_RS26420 94816..95298 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
HH204_RS26425 95719..96946 + 1228 Protein_99 IS3 family transposase -
HH204_RS26430 96942..97337 - 396 Protein_100 DDE-type integrase/transposase/recombinase -
HH204_RS26435 97512..98534 - 1023 WP_004214536.1 porphobilinogen synthase -
HH204_RS26440 98598..99944 - 1347 WP_011154555.1 dihydroorotase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA2 / iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroN / rmpA / rmpA 1..207560 207560
- inside IScluster/Tn - - 89794..96946 7152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T155418 WP_004098919.1 NZ_CP052037:94816-95298 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T155418 NZ_CP068801:2250694-2250797 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT155418 WP_004213599.1 NZ_CP052037:94562-94828 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT155418 NZ_CP068801:c2250835-2250564 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References