Detailed information of TA system TA155417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 28702..29372
• Replicon: plasmid pKPN41953
• Accession: NZ_CP052037
• Organism: Klebsiella pneumoniae strain KPN41053

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: HH204_RS26085
• Protein ID: WP_004213072.1
• Coordinates: 28929..29372 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: HH204_RS26080
• Protein ID: WP_004213073.1
• Coordinates: 28702..28932 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HH204_RS26055	24925..25824	-	900	WP_004225022.1	nucleotide-binding protein	-
HH204_RS26060	25814..26104	-	291	WP_004213078.1	hypothetical protein	-
HH204_RS26065	26456..26662	+	207	WP_004213077.1	hypothetical protein	-
HH204_RS26070	26652..26945	-	294	WP_004213076.1	hypothetical protein	-
HH204_RS26075	26961..28094	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
HH204_RS26080	28702..28932	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HH204_RS26085	28929..29372	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
HH204_RS26090	29386..29802	-	417	Protein_32	restriction endonuclease	-
HH204_RS26095	29806..30099	-	294	Protein_33	transposase	-
HH204_RS26100	30516..31152	+	637	Protein_34	response regulator	-
HH204_RS26105	31667..32073	-	407	Protein_35	GAF domain-containing protein	-
HH204_RS26110	32164..33084	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
HH204_RS26115	33133..33624	-	492	WP_011251327.1	DM13 domain-containing protein	-
HH204_RS26120	33687..33962	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroN / rmpA / rmpA	1..207560	207560

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T155417 WP_004213072.1 NZ_CP052037:28929-29372 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T155417 NZ_CP068801:c2055196-2054822 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT155417 WP_004213073.1 NZ_CP052037:28702-28932 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT155417 NZ_CP068801:c2055653-2055285 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620