Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 834940..835565 Replicon chromosome
Accession NZ_CP052036
Organism Klebsiella pneumoniae strain KPN41053

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HH204_RS03935 Protein ID WP_002882817.1
Coordinates 834940..835323 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HH204_RS03940 Protein ID WP_004150355.1
Coordinates 835323..835565 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HH204_RS03920 832306..833208 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HH204_RS03925 833205..833840 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HH204_RS03930 833837..834766 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HH204_RS03935 834940..835323 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HH204_RS03940 835323..835565 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HH204_RS03945 835770..836687 + 918 WP_088497967.1 alpha/beta hydrolase -
HH204_RS03950 836701..837642 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HH204_RS03955 837687..838124 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HH204_RS03960 838121..838981 - 861 WP_002882807.1 virulence factor BrkB family protein -
HH204_RS03965 838975..839574 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T155402 WP_002882817.1 NZ_CP052036:c835323-834940 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T155402 NZ_CP068800:c4675887-4675714 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT155402 WP_004150355.1 NZ_CP052036:c835565-835323 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT155402 NZ_CP068800:c4676211-4675915 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References