Detailed information of TA system TA155397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 40964..41548
• Replicon: plasmid pAb-B004d-c_1
• Accession: NZ_CP051876
• Organism: Acinetobacter baumannii strain Ab-B004d-c

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: HIN86_RS20055
• Protein ID: WP_000286964.1
• Coordinates: 41246..41548 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HIN86_RS20050
• Protein ID: WP_000985609.1
• Coordinates: 40964..41245 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HIN86_RS20030	36707..37042	+	336	WP_169031081.1	hypothetical protein	-
HIN86_RS20035	37255..37866	+	612	WP_015060246.1	recombinase family protein	-
HIN86_RS20040	38056..38940	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
HIN86_RS20045	38996..40471	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
HIN86_RS20050	40964..41245	-	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
HIN86_RS20055	41246..41548	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HIN86_RS20060	41703..42344	-	642	WP_004787586.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
HIN86_RS20065	42410..43597	+	1188	WP_004856455.1	tetracycline efflux MFS transporter Tet(39)	-
HIN86_RS20075	44108..44674	+	567	WP_004812033.1	recombinase family protein	-
HIN86_RS20080	45045..45749	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA1 / mph(E) / msr(E) / tet(39) / aac(3)-IId	-	1..48239	48239
	-	inside	IScluster/Tn	mph(E) / msr(E) / tet(39) / aac(3)-IId	-	38056..48187	10131
	-	inside	Integron	-	-	53..48187	48134

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T155397 WP_000286964.1 NZ_CP051876:c41548-41246 [Acinetobacter baumannii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T155397 NZ_CP068800:c4075055-4074657 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT155397 WP_000985609.1 NZ_CP051876:c41245-40964 [Acinetobacter baumannii]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT155397 NZ_CP068800:c4075351-4075058 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94