Detailed information of TA system TA155387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 34030..34614
• Replicon: plasmid pAb-C63_1
• Accession: NZ_CP051867
• Organism: Acinetobacter baumannii strain Ab-C63

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: HIN88_RS18660
• Protein ID: WP_000286964.1
• Coordinates: 34030..34332 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HIN88_RS18665
• Protein ID: WP_000985609.1
• Coordinates: 34333..34614 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HIN88_RS18640	29894..30658	-	765	Protein_18	IS3-like element ISAba19 family transposase	-
HIN88_RS18645	31172..31738	+	567	WP_000678311.1	recombinase family protein	-
HIN88_RS18650	31981..33168	-	1188	WP_004856455.1	tetracycline efflux MFS transporter Tet(39)	-
HIN88_RS18655	33234..33875	+	642	WP_004787586.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
HIN88_RS18660	34030..34332	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HIN88_RS18665	34333..34614	+	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
HIN88_RS18670	34694..35113	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
HIN88_RS18675	35253..35573	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HIN88_RS18680	35566..35838	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
HIN88_RS18685	36331..37806	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
HIN88_RS18690	37862..38746	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
HIN88_RS18695	38936..39547	-	612	WP_015060246.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(39) / msr(E) / mph(E) / aph(3')-Ia / ant(2'')-Ia / blaOXA-420 / aph(3'')-Ib / aph(6)-Id / sul2 / dfrA20	-	1..81353	81353
	-	inside	IScluster/Tn	tet(39) / msr(E) / mph(E) / aph(3')-Ia / ant(2'')-Ia / blaOXA-420 / aph(3'')-Ib / aph(6)-Id / sul2 / dfrA20	-	29894..59209	29315

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T155387 WP_000286964.1 NZ_CP051867:34030-34332 [Acinetobacter baumannii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T155387 NZ_CP068800:1278618-1278929 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT155387 WP_000985609.1 NZ_CP051867:34333-34614 [Acinetobacter baumannii]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT155387 NZ_CP068800:1278926-1279345 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94