Detailed information of TA system TA155385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 16562..17090
• Replicon: plasmid pAb-C102_3
• Accession: NZ_CP051865
• Organism: Acinetobacter baumannii strain Ab-C102

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9N871
• Locus tag: HIN89_RS18750
• Protein ID: WP_000221358.1
• Coordinates: 16797..17090 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: N9NWT9
• Locus tag: HIN89_RS18745
• Protein ID: WP_000246755.1
• Coordinates: 16562..16807 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HIN89_RS18710	12367..12891	+	525	WP_005127114.1	plasmid replication DNA-binding protein	-
HIN89_RS18715	13088..13348	-	261	WP_004698336.1	hypothetical protein	-
HIN89_RS18720	13515..13835	+	321	WP_004698338.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HIN89_RS18725	13828..14100	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
HIN89_RS18730	14190..15515	-	1326	WP_016805832.1	ISNCY family transposase	-
HIN89_RS18735	15665..16063	-	399	WP_024437500.1	hypothetical protein	-
HIN89_RS18740	16085..16498	-	414	WP_086266947.1	DUF3800 domain-containing protein	-
HIN89_RS18745	16562..16807	+	246	WP_000246755.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HIN89_RS18750	16797..17090	+	294	WP_000221358.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HIN89_RS18755	17128..17400	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
HIN89_RS18760	17393..17713	-	321	WP_004698338.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HIN89_RS18765	17879..18139	+	261	WP_004698336.1	hypothetical protein	-
HIN89_RS18770	18310..19635	+	1326	WP_016805832.1	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..19853	19853
	-	inside	IScluster/Tn	-	-	4260..19635	15375

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-72)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11292.52 Da
• Isoelectric Point: 10.4753

>T155385 WP_000221358.1 NZ_CP051865:16797-17090 [Acinetobacter baumannii]
MTYKLLRHKDFTAEWEKLPVAIRDQFKKKLAKVIEQPHIPKNMLRGDLAGCYKIKLLKAGVRLVYQVKDDQVVILLITVG
KRADSIVYDEAKKRIKD

Nucleotide

• Length: 294 bp

>T155385 NZ_CP068800:1045518-1045925 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9249.64 Da
• Isoelectric Point: 4.3142

>AT155385 WP_000246755.1 NZ_CP051865:16562-16807 [Acinetobacter baumannii]
MATVNFRIDEALKEKSYSILKEQGIAPTDFFTSILEYVATTGKLPVKKALLSEEDEELLALVRKRINDPKEMFEEVTLDD
L

Nucleotide

• Length: 246 bp

>AT155385 NZ_CP068800:1045271-1045537 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGATGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A6A1RKP4

Antitoxin

• AlphaFold DB: A0A6A1RJ80