155361

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1380142..1381058 Replicon chromosome
Accession NZ_CP051860
Organism Bacillus subtilis subsp. subtilis str. 168

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag HIR76_RS07225 Protein ID WP_003244695.1
Coordinates 1380312..1381058 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag HIR76_RS07220 Protein ID WP_003232646.1
Coordinates 1380142..1380312 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HIR76_RS07185 1377005..1377334 + 330 WP_003232660.1 phage-like element PBSX protein XkdW -
HIR76_RS07190 1377331..1377495 + 165 WP_003232658.1 XkdX family protein -
HIR76_RS07195 1377539..1378378 + 840 WP_003245597.1 phage-like element PBSX protein XepA -
HIR76_RS07200 1378431..1378700 + 270 WP_003232655.1 hemolysin XhlA family protein -
HIR76_RS07205 1378713..1378976 + 264 WP_003232653.1 phage holin -
HIR76_RS07210 1378989..1379882 + 894 WP_003245230.1 N-acetylmuramoyl-L-alanine amidase -
HIR76_RS07215 1379919..1380056 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
HIR76_RS07220 1380142..1380312 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
HIR76_RS07225 1380312..1381058 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
HIR76_RS07230 1381168..1382169 - 1002 WP_003232642.1 inorganic phosphate transporter -
HIR76_RS07235 1382182..1382799 - 618 WP_003218470.1 DUF47 domain-containing protein -
HIR76_RS07240 1383075..1384391 - 1317 WP_003244819.1 serine/threonine exchanger -
HIR76_RS07245 1384780..1385730 + 951 WP_003245086.1 ring-cleaving dioxygenase -
HIR76_RS07250 1385831..1385977 + 147 WP_003244977.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T155361 WP_003244695.1 NZ_CP051860:c1381058-1380312 [Bacillus subtilis subsp. subtilis str. 168]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T155361 NZ_CP068799:2283860-2283962 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT155361 WP_003232646.1 NZ_CP051860:c1380312-1380142 [Bacillus subtilis subsp. subtilis str. 168]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT155361 NZ_CP068799:c2284000-2283732 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References