Detailed information of TA system TA155361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1380142..1381058
• Replicon: chromosome
• Accession: NZ_CP051860
• Organism: Bacillus subtilis subsp. subtilis str. 168

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: O34853
• Locus tag: HIR76_RS07225
• Protein ID: WP_003244695.1
• Coordinates: 1380312..1381058 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: HIR76_RS07220
• Protein ID: WP_003232646.1
• Coordinates: 1380142..1380312 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HIR76_RS07185	1377005..1377334	+	330	WP_003232660.1	phage-like element PBSX protein XkdW	-
HIR76_RS07190	1377331..1377495	+	165	WP_003232658.1	XkdX family protein	-
HIR76_RS07195	1377539..1378378	+	840	WP_003245597.1	phage-like element PBSX protein XepA	-
HIR76_RS07200	1378431..1378700	+	270	WP_003232655.1	hemolysin XhlA family protein	-
HIR76_RS07205	1378713..1378976	+	264	WP_003232653.1	phage holin	-
HIR76_RS07210	1378989..1379882	+	894	WP_003245230.1	N-acetylmuramoyl-L-alanine amidase	-
HIR76_RS07215	1379919..1380056	-	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
HIR76_RS07220	1380142..1380312	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
HIR76_RS07225	1380312..1381058	-	747	WP_003244695.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
HIR76_RS07230	1381168..1382169	-	1002	WP_003232642.1	inorganic phosphate transporter	-
HIR76_RS07235	1382182..1382799	-	618	WP_003218470.1	DUF47 domain-containing protein	-
HIR76_RS07240	1383075..1384391	-	1317	WP_003244819.1	serine/threonine exchanger	-
HIR76_RS07245	1384780..1385730	+	951	WP_003245086.1	ring-cleaving dioxygenase	-
HIR76_RS07250	1385831..1385977	+	147	WP_003244977.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.50 Da
• Isoelectric Point: 4.6191

>T155361 WP_003244695.1 NZ_CP051860:c1381058-1380312 [Bacillus subtilis subsp. subtilis str. 168]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T155361 NZ_CP068799:2283860-2283962 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT155361 WP_003232646.1 NZ_CP051860:c1380312-1380142 [Bacillus subtilis subsp. subtilis str. 168]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT155361 NZ_CP068799:c2284000-2283732 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• PDB: 3O6Q

Antitoxin

• AlphaFold DB: G4EXD8