Detailed information of TA system TA155360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 518665..519301
• Replicon: chromosome
• Accession: NZ_CP051860
• Organism: Bacillus subtilis subsp. subtilis str. 168

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: HIR76_RS02625
• Protein ID: WP_003156187.1
• Coordinates: 518951..519301 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: HIR76_RS02620
• Protein ID: WP_003225183.1
• Coordinates: 518665..518946 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HIR76_RS02600	515024..515623	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
HIR76_RS02605	515718..516083	+	366	WP_003234281.1	holo-ACP synthase	-
HIR76_RS02610	516249..517265	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
HIR76_RS02615	517380..518549	+	1170	WP_003234284.1	alanine racemase	-
HIR76_RS02620	518665..518946	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
HIR76_RS02625	518951..519301	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
HIR76_RS02630	519416..520240	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
HIR76_RS02635	520245..520610	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
HIR76_RS02640	520614..521015	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
HIR76_RS02645	521027..522034	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
HIR76_RS02650	522096..522425	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
HIR76_RS02655	522422..522904	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
HIR76_RS02660	522870..523658	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
HIR76_RS02665	523658..524257	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T155360 WP_003156187.1 NZ_CP051860:518951-519301 [Bacillus subtilis subsp. subtilis str. 168]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T155360 NZ_CP068799:c1995428-1995054 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT155360 WP_003225183.1 NZ_CP051860:518665-518946 [Bacillus subtilis subsp. subtilis str. 168]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT155360 NZ_CP068799:c1995885-1995517 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAACGTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32