Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4445716..4446266 Replicon chromosome
Accession NZ_CP051849
Organism Escherichia coli strain SCU-101

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag HHJ20_RS21625 Protein ID WP_001160966.1
Coordinates 4445716..4446030 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HHJ20_RS21630 Protein ID WP_000125566.1
Coordinates 4446033..4446266 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ20_RS21600 (4441391) 4441391..4442677 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
HHJ20_RS21605 (4442677) 4442677..4443666 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HHJ20_RS21610 (4443663) 4443663..4444484 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HHJ20_RS21615 (4444487) 4444487..4444864 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HHJ20_RS21620 (4444871) 4444871..4445719 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HHJ20_RS21625 (4445716) 4445716..4446030 - 315 WP_001160966.1 CcdB family protein Toxin
HHJ20_RS21630 (4446033) 4446033..4446266 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HHJ20_RS21635 (4446352) 4446352..4446831 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
HHJ20_RS21640 (4447023) 4447023..4448885 - 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
HHJ20_RS21645 (4448878) 4448878..4449408 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HHJ20_RS21650 (4449516) 4449516..4450847 - 1332 WP_001183207.1 MFS transporter -
HHJ20_RS21655 (4450906) 4450906..4451193 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T155348 WP_001160966.1 NZ_CP051849:c4446030-4445716 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T155348 NZ_CP068798:4169820-4170038 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT155348 WP_000125566.1 NZ_CP051849:c4446266-4446033 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT155348 NZ_CP068798:4169419-4169793 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References