Detailed information of TA system TA155335

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2155115..2155946
• Replicon: chromosome
• Accession: NZ_CP051849
• Organism: Escherichia coli strain SCU-101

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: HHJ20_RS10415
• Protein ID: WP_000854814.1
• Coordinates: 2155115..2155489 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HHJ20_RS10420
• Protein ID: WP_001560600.1
• Coordinates: 2155578..2155946 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ20_RS10375 (2150511)	2150511..2151677	+	1167	WP_001296209.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
HHJ20_RS10380 (2151796)	2151796..2152269	+	474	WP_001560602.1	DNA gyrase inhibitor SbmC	-
HHJ20_RS10385 (2152467)	2152467..2153525	+	1059	WP_001560601.1	FUSC family protein	-
HHJ20_RS10390 (2153697)	2153697..2154026	+	330	WP_000450409.1	DUF496 family protein	-
HHJ20_RS26140 (2154127)	2154127..2154483	-	357	WP_000929389.1	EutP/PduV family microcompartment system protein	-
HHJ20_RS10405 (2154798)	2154798..2154878	-	81	Protein_2059	hypothetical protein	-
HHJ20_RS10410 (2154924)	2154924..2155118	-	195	WP_000988600.1	DUF5983 family protein	-
HHJ20_RS10415 (2155115)	2155115..2155489	-	375	WP_000854814.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
HHJ20_RS10420 (2155578)	2155578..2155946	-	369	WP_001560600.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
HHJ20_RS10425 (2156020)	2156020..2156241	-	222	WP_000692315.1	DUF987 domain-containing protein	-
HHJ20_RS10430 (2156304)	2156304..2156780	-	477	WP_001186170.1	RadC family protein	-
HHJ20_RS10435 (2156796)	2156796..2157269	-	474	WP_001313575.1	antirestriction protein	-
HHJ20_RS10440 (2157532)	2157532..2158353	-	822	WP_021561125.1	DUF932 domain-containing protein	-
HHJ20_RS10445 (2158574)	2158574..2158984	-	411	WP_000846704.1	hypothetical protein	-
HHJ20_RS10450 (2159000)	2159000..2159678	-	679	Protein_2068	hypothetical protein	-
HHJ20_RS10455 (2159814)	2159814..2160884	-	1071	WP_000102631.1	patatin-like phospholipase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T155335 WP_000854814.1 NZ_CP051849:c2155489-2155115 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T155335 NZ_CP068797:2082617-2082719 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13503.31 Da
• Isoelectric Point: 5.9526

>AT155335 WP_001560600.1 NZ_CP051849:c2155946-2155578 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLANRAGIRGLSSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLACEADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT155335 NZ_CP068797:c2082726-2082581 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A829FY50