Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1108770..1109424 Replicon chromosome
Accession NZ_CP051849
Organism Escherichia coli strain SCU-101

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HHJ20_RS05445 Protein ID WP_000244781.1
Coordinates 1109017..1109424 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HHJ20_RS05440 Protein ID WP_000354046.1
Coordinates 1108770..1109036 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ20_RS05420 (1104858) 1104858..1106291 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
HHJ20_RS05425 (1106336) 1106336..1106647 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
HHJ20_RS05430 (1106811) 1106811..1107470 + 660 WP_000250274.1 hemolysin III family protein -
HHJ20_RS05435 (1107547) 1107547..1108527 - 981 WP_000886079.1 tRNA-modifying protein YgfZ -
HHJ20_RS05440 (1108770) 1108770..1109036 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HHJ20_RS05445 (1109017) 1109017..1109424 + 408 WP_000244781.1 protein YgfX Toxin
HHJ20_RS05450 (1109464) 1109464..1109985 - 522 WP_001055874.1 flavodoxin FldB -
HHJ20_RS05455 (1110097) 1110097..1110993 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HHJ20_RS05460 (1111018) 1111018..1111728 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HHJ20_RS05465 (1111734) 1111734..1113467 + 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T155332 WP_000244781.1 NZ_CP051849:1109017-1109424 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T155332 NZ_CP068796:c4982648-4982337 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT155332 WP_000354046.1 NZ_CP051849:1108770-1109036 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT155332 NZ_CP068796:c4982336-4982046 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References