Detailed information of TA system TA155324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 2987448..2988121
• Replicon: chromosome
• Accession: NZ_CP051772
• Organism: Mesorhizobium japonicum R7A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: R7A2020_RS14720
• Protein ID: WP_032930876.1
• Coordinates: 2987723..2988121 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6I4AZ99
• Locus tag: R7A2020_RS14715
• Protein ID: WP_032930877.1
• Coordinates: 2987448..2987723 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
R7A2020_RS14690	2982828..2983808	+	981	WP_032930881.1	ABC transporter permease	-
R7A2020_RS14695	2983805..2984791	+	987	WP_032930880.1	ABC transporter permease	-
R7A2020_RS14700	2984907..2985455	+	549	WP_032930879.1	gluconokinase	-
R7A2020_RS14705	2985539..2985988	-	450	WP_010910348.1	Rrf2 family transcriptional regulator	-
R7A2020_RS14710	2986237..2987223	-	987	WP_032930878.1	sulfate ABC transporter substrate-binding protein	-
R7A2020_RS14715	2987448..2987723	+	276	WP_032930877.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
R7A2020_RS14720	2987723..2988121	+	399	WP_032930876.1	type II toxin-antitoxin system VapC family toxin	Toxin
R7A2020_RS14725	2988137..2989177	-	1041	WP_010910344.1	sulfate/molybdate ABC transporter ATP-binding protein	-
R7A2020_RS14730	2989190..2990074	-	885	WP_032930875.1	sulfate ABC transporter permease subunit CysW	-
R7A2020_RS14735	2990067..2990918	-	852	WP_010910342.1	sulfate ABC transporter permease subunit CysT	-
R7A2020_RS14740	2991000..2992028	-	1029	WP_032930874.1	sulfate ABC transporter substrate-binding protein	-
R7A2020_RS14745	2992233..2992964	-	732	WP_080680275.1	M15 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

• SpoVT-AbrB domain (18-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14248.63 Da
• Isoelectric Point: 5.7489

>T155324 WP_032930876.1 NZ_CP051772:2987723-2988121 [Mesorhizobium japonicum R7A]
MRFMLDTNIISDMIRNPAGKAAGAMVREGDAAVCTSIVVASELRYGCARKGSTKLLKKVEDLLAEIPVLPLDVPVDAEYG
ALRAELEAVGQPIGHNDLFIAAHACVLGTTLVTANIGEFTRIRKLKVENWLE

Nucleotide

• Length: 399 bp

>T155324 NZ_CP068796:3847140-3847358 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.65 Da
• Isoelectric Point: 4.8605

>AT155324 WP_032930877.1 NZ_CP051772:2987448-2987723 [Mesorhizobium japonicum R7A]
MPQHRQTTTPPPKEAKLFRNNRSQAVRIPVEFELPGDKVLISREGDRLVIEPLRKPGLAALLAQWAKEAPLGPEDSFPEI
NDAPVEAEDIF

Nucleotide

• Length: 276 bp

>AT155324 NZ_CP068796:3846740-3847114 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A6I4AZ99