Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB-HicA
Location 2643831..2644426 Replicon chromosome
Accession NZ_CP051772
Organism Mesorhizobium japonicum R7A

Toxin (Protein)


Gene name hicA Uniprot ID A0A4Q1UJ83
Locus tag R7A2020_RS12950 Protein ID WP_065141637.1
Coordinates 2644238..2644426 (-) Length 63 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q98J17
Locus tag R7A2020_RS12945 Protein ID WP_010910701.1
Coordinates 2643831..2644232 (-) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
R7A2020_RS12920 2639126..2640421 - 1296 WP_032931139.1 TIGR01244 family phosphatase -
R7A2020_RS12925 2640797..2641249 + 453 WP_032931138.1 SRPBCC domain-containing protein -
R7A2020_RS12930 2641278..2642108 - 831 WP_032931136.1 helix-turn-helix transcriptional regulator -
R7A2020_RS12935 2642199..2643083 + 885 WP_032931134.1 SDR family oxidoreductase -
R7A2020_RS12940 2643096..2643794 - 699 WP_032931133.1 RibD family protein -
R7A2020_RS12945 2643831..2644232 - 402 WP_010910701.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
R7A2020_RS12950 2644238..2644426 - 189 WP_065141637.1 type II toxin-antitoxin system HicA family toxin Toxin
R7A2020_RS12955 2644787..2645734 + 948 WP_032931132.1 substrate-binding domain-containing protein -
R7A2020_RS12960 2645977..2647575 + 1599 WP_032931131.1 sugar ABC transporter ATP-binding protein -
R7A2020_RS12965 2647572..2648588 + 1017 WP_010910697.1 ABC transporter permease -
R7A2020_RS12970 2648623..2649129 - 507 WP_080680478.1 histidine phosphatase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-124)


Sequences

Toxin        


Download         Length: 63 a.a.        Molecular weight: 7148.23 Da        Isoelectric Point: 11.6118

>T155323 WP_065141637.1 NZ_CP051772:c2644426-2644238 [Mesorhizobium japonicum R7A]
MNSTEIIRQLQADGWFEVARKGSHAQFKHRDKQGRVTVPHPKRDIPIGTLRSIEKQSGLKLR

Download         Length: 189 bp

>T155323 NZ_CP068796:3444199-3444585 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14185.00 Da        Isoelectric Point: 4.3919

>AT155323 WP_010910701.1 NZ_CP051772:c2644232-2643831 [Mesorhizobium japonicum R7A]
MKQYIGLIHKDAGSDFGVSFPDFPGVITAGKDLDDARSMAEEALTLHIEGLVEDGEAIPEPSSLQAVMADADNRDGVAIL
VAAKTEARKAVRINVTLPDDVLRRIDAFAEAHGYTRSGFLAKAAEKAMELESA

Download         Length: 402 bp

>AT155323 NZ_CP068796:3444575-3444904 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Q1UJ83

Antitoxin

Source ID Structure
AlphaFold DB A0A4Q1UK54

References