Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4287333..4287830 Replicon chromosome
Accession NZ_CP051765
Organism Escherichia coli strain SCU-113

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HHJ30_RS20495 Protein ID WP_001173343.1
Coordinates 4287333..4287506 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HHJ30_RS20500 Protein ID WP_000398619.1
Coordinates 4287534..4287830 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ30_RS20485 4284003..4285460 + 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -
HHJ30_RS20490 4285697..4287214 + 1518 WP_001295074.1 lysine--tRNA ligase -
HHJ30_RS20495 4287333..4287506 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HHJ30_RS20500 4287534..4287830 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HHJ30_RS20505 4288057..4288329 - 273 WP_000405647.1 N-acetyltransferase -
HHJ30_RS20510 4288341..4288571 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
HHJ30_RS20515 4288752..4290383 + 1632 WP_114747508.1 sensor histidine kinase -
HHJ30_RS20520 4290380..4291099 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T155321 WP_001173343.1 NZ_CP051765:c4287506-4287333 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T155321 NZ_CP068796:3008772-3008879 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACTTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT155321 WP_000398619.1 NZ_CP051765:c4287830-4287534 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT155321 NZ_CP068796:c3008724-3008658 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References