Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4027726..4028302 Replicon chromosome
Accession NZ_CP051765
Organism Escherichia coli strain SCU-113

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag HHJ30_RS19235 Protein ID WP_001350372.1
Coordinates 4028015..4028302 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1X0YJR8
Locus tag HHJ30_RS19230 Protein ID WP_001402821.1
Coordinates 4027726..4028028 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ30_RS19210 4023417..4023929 + 513 WP_001175456.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
HHJ30_RS19215 4024322..4026472 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
HHJ30_RS19220 4026522..4026725 + 204 WP_000467859.1 YbdD/YjiX family protein -
HHJ30_RS19225 4026736..4027692 + 957 WP_001295525.1 GTPase -
HHJ30_RS19230 4027726..4028028 - 303 WP_001402821.1 BrnA antitoxin family protein Antitoxin
HHJ30_RS19235 4028015..4028302 - 288 WP_001350372.1 BrnT family toxin Toxin
HHJ30_RS19240 4028499..4029665 + 1167 WP_001672354.1 restriction endonuclease -
HHJ30_RS19245 4029729..4031348 + 1620 WP_001029742.1 type I restriction-modification system subunit M -
HHJ30_RS19250 4031338..4032720 + 1383 WP_023155808.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T155319 WP_001350372.1 NZ_CP051765:c4028302-4028015 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T155319 NZ_CP068796:c2754958-2754782 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11534.04 Da        Isoelectric Point: 9.4646

>AT155319 WP_001402821.1 NZ_CP051765:c4028028-4027726 [Escherichia coli]
MSMVKHKRGTSSILDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT155319 NZ_CP068796:c2754736-2754320 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATCAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A1X0YJR8

References