Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1309673..1310298 Replicon chromosome
Accession NZ_CP051765
Organism Escherichia coli strain SCU-113

Toxin (Protein)


Gene name vapC Uniprot ID A0A2J1DF47
Locus tag HHJ30_RS06285 Protein ID WP_000911331.1
Coordinates 1309900..1310298 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag HHJ30_RS06280 Protein ID WP_000450524.1
Coordinates 1309673..1309900 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ30_RS06255 1305475..1305945 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
HHJ30_RS06260 1305945..1306517 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
HHJ30_RS06265 1306663..1307541 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
HHJ30_RS06270 1307558..1308592 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
HHJ30_RS06275 1308805..1309518 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
HHJ30_RS06280 1309673..1309900 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
HHJ30_RS06285 1309900..1310298 + 399 WP_000911331.1 tRNA(fMet)-specific endonuclease VapC Toxin
HHJ30_RS06290 1310445..1311308 + 864 WP_001267507.1 neutral zinc metallopeptidase -
HHJ30_RS06295 1311323..1313338 + 2016 WP_001543081.1 tRNA cytosine(34) acetyltransferase TmcA -
HHJ30_RS06300 1313412..1314110 + 699 WP_000679812.1 esterase -
HHJ30_RS06305 1314220..1314420 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14862.19 Da        Isoelectric Point: 9.2216

>T155305 WP_000911331.1 NZ_CP051765:1309900-1310298 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLVELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T155305 NZ_CP068795:c4314249-4313899 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT155305 WP_000450524.1 NZ_CP051765:1309673-1309900 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT155305 NZ_CP068795:c4314494-4314243 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J1DF47

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References