Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3676112..3676766 Replicon chromosome
Accession NZ_CP051753
Organism Escherichia coli strain SCU-102

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HHJ21_RS17325 Protein ID WP_000244781.1
Coordinates 3676112..3676519 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HHJ21_RS17330 Protein ID WP_000354046.1
Coordinates 3676500..3676766 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ21_RS17305 3672069..3673802 - 1734 WP_000813188.1 single-stranded-DNA-specific exonuclease RecJ -
HHJ21_RS17310 3673808..3674518 - 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HHJ21_RS17315 3674543..3675439 - 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
HHJ21_RS17320 3675551..3676072 + 522 WP_001055874.1 flavodoxin FldB -
HHJ21_RS17325 3676112..3676519 - 408 WP_000244781.1 protein YgfX Toxin
HHJ21_RS17330 3676500..3676766 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HHJ21_RS17335 3677009..3677989 + 981 WP_000886074.1 tRNA-modifying protein YgfZ -
HHJ21_RS17340 3678066..3678725 - 660 WP_000250268.1 hemolysin III family protein -
HHJ21_RS17345 3678889..3679200 - 312 WP_001182963.1 N(4)-acetylcytidine aminohydrolase -
HHJ21_RS17350 3679245..3680678 + 1434 WP_024177858.1 6-phospho-beta-glucosidase BglA -
HHJ21_RS17355 3680740..3681483 - 744 WP_001366570.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T155297 WP_000244781.1 NZ_CP051753:c3676519-3676112 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T155297 NZ_CP068795:c2475073-2474897 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT155297 WP_000354046.1 NZ_CP051753:c3676766-3676500 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT155297 NZ_CP068795:c2474851-2474435 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References