Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4829918..4830534 Replicon chromosome
Accession NZ_CP051751
Organism Escherichia coli strain SCU-301

Toxin (Protein)


Gene name vapC Uniprot ID A0A176ZIX8
Locus tag HHJ43_RS22965 Protein ID WP_001129490.1
Coordinates 4829918..4830292 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1M0CY48
Locus tag HHJ43_RS22970 Protein ID WP_001275523.1
Coordinates 4830292..4830534 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ43_RS22950 4827421..4828323 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
HHJ43_RS22955 4828320..4828955 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
HHJ43_RS22960 4828952..4829881 + 930 WP_096986988.1 formate dehydrogenase accessory protein FdhE -
HHJ43_RS22965 4829918..4830292 - 375 WP_001129490.1 type II toxin-antitoxin system VapC family toxin Toxin
HHJ43_RS22970 4830292..4830534 - 243 WP_001275523.1 ribbon-helix-helix domain-containing protein Antitoxin
HHJ43_RS22975 4830753..4830971 - 219 WP_001295676.1 ribbon-helix-helix domain-containing protein -
HHJ43_RS22980 4831667..4833481 - 1815 WP_096986989.1 hypothetical protein -
HHJ43_RS22985 4833897..4834838 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
HHJ43_RS22990 4834883..4835320 - 438 WP_096986990.1 D-tyrosyl-tRNA(Tyr) deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-48)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.19 Da        Isoelectric Point: 7.0126

>T155275 WP_001129490.1 NZ_CP051751:c4830292-4829918 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

>T155275 NZ_CP068771:1771990-1772086 [Serratia plymuthica]
AACAAGCCCTGCACAAAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATC
GTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9290.54 Da        Isoelectric Point: 4.3774

>AT155275 WP_001275523.1 NZ_CP051751:c4830534-4830292 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT155275 NZ_CP068771:c1772088-1771946 [Serratia plymuthica]
ATAAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTTTGTGCAGGGCTTGTTCAGCCATGCACTTTAAGAGTAGCTTACCGCGCTAGTTTTGCCAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A176ZIX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CY48

References