Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4527064..4527561 Replicon chromosome
Accession NZ_CP051751
Organism Escherichia coli strain SCU-301

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HHJ43_RS21630 Protein ID WP_001173343.1
Coordinates 4527064..4527237 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HHJ43_RS21635 Protein ID WP_000398619.1
Coordinates 4527265..4527561 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ43_RS21620 4523734..4525191 + 1458 WP_000856843.1 dipeptide/tripeptide permease DtpC -
HHJ43_RS21625 4525428..4526945 + 1518 WP_000003806.1 lysine--tRNA ligase -
HHJ43_RS21630 4527064..4527237 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HHJ43_RS21635 4527265..4527561 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HHJ43_RS21640 4527790..4528062 - 273 WP_000405647.1 N-acetyltransferase -
HHJ43_RS21645 4528074..4528304 - 231 WP_000371713.1 (4Fe-4S)-binding protein -
HHJ43_RS21650 4528485..4530116 + 1632 WP_001216448.1 sensor histidine kinase -
HHJ43_RS21655 4530113..4530832 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T155274 WP_001173343.1 NZ_CP051751:c4527237-4527064 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T155274 NZ_CP068771:1452301-1452555 [Serratia plymuthica]
ATGAACAAACGACACCAAAAAACGCTGTCAGATGTGTTTGCCCGACCTGTCAACGGTTCTATAAAGTGGTCTGATATTGA
GGCGCTTTTTACCGCATTAGGGGCGGAGATTCACGAAAGGGAAGGTTCTAGGATCGCGGTGTTGTTGAAAGATGAAAAAC
GTGTCTTTCACCGGCCACACCCCAGACCTACCACTGACAAGGGGGCGGTTAACTCCATACGGATCTGGTTGGATAGCTTA
GGAATTAAACCATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT155274 WP_000398619.1 NZ_CP051751:c4527561-4527265 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT155274 NZ_CP068771:1452552-1452896 [Serratia plymuthica]
ATGATGAACAATACACTTAAAATCGACGGCCACGTGGCCGTTATTACCTTTGACCCTGAAATTGAAATGTTCCGGGGTGA
GTTTGTCGGACTGAATGGCGGTGCTGACTTCTATGCCTACAGTGTTGAAGAACTGAAAACAGAAGGCTCTACCTCGCTTG
CGATCTTTTTAGATGAGTGTGAAAAAGATGGTATTGCACCTTACAAATCGTATAGCGGCAAAGTGACCACTCGCTTGTCG
CCAGAGCGTCATCAGGCATTAGCGATTGCGGCACAAGCCACCGGGCAGTCAATCAATGAGCTGCTGAATGAAGGTGTTGA
TTTGGTAATCGAAAAACATTCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References