Detailed information of TA system TA155272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4116814..4117364
• Replicon: chromosome
• Accession: NZ_CP051751
• Organism: Escherichia coli strain SCU-301

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: HHJ43_RS19670
• Protein ID: WP_096987052.1
• Coordinates: 4116814..4117128 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1NMQ0
• Locus tag: HHJ43_RS19675
• Protein ID: WP_000125568.1
• Coordinates: 4117131..4117364 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ43_RS19645	4112489..4113775	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
HHJ43_RS19650	4113775..4114764	+	990	WP_088539631.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
HHJ43_RS19655	4114761..4115582	+	822	WP_075861932.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
HHJ43_RS19660	4115585..4115962	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
HHJ43_RS19665	4115969..4116817	+	849	WP_000257195.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
HHJ43_RS19670	4116814..4117128	-	315	WP_096987052.1	CcdB family protein	Toxin
HHJ43_RS19675	4117131..4117364	-	234	WP_000125568.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HHJ43_RS19680	4117450..4117929	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
HHJ43_RS19685	4118121..4119983	-	1863	WP_000377117.1	glutathione-regulated potassium-efflux system protein KefC	-
HHJ43_RS19690	4119976..4120506	-	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
HHJ43_RS19695	4120614..4121945	-	1332	WP_001183201.1	MFS transporter	-
HHJ43_RS19700	4122003..4122290	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11658.67 Da
• Isoelectric Point: 7.8608

>T155272 WP_096987052.1 NZ_CP051751:c4117128-4116814 [Escherichia coli]
MQFTVYCSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T155272 NZ_CP068771:c697667-697377 [Serratia plymuthica]
GTGCCACAACTGATATGGACGCCAGCGGCGCTGCTGGATGTGCAACGTCTTTATCGATTTCTGGCCGAGAAGGATCTCAA
TTCGGCAAAGCGCGCTGTCGCCGCTATTCGCAACAGCATCAAAATAATAGCGCACCAACCCAATACTGGCCGCCCCGCTA
AAGATATGGCACCTGAATTCAGGGAGTGGCCTGTCGATTTCGGTAATAGCGGTTATCTTGTTCTGTATCGGTTTAACGGT
GTAACCGCGGTTATTTTGGCTATTCGCCACCAAAGTGAAACCGGGTATTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8560.55 Da
• Isoelectric Point: 5.8062

>AT155272 WP_000125568.1 NZ_CP051751:c4117364-4117131 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT155272 NZ_CP068771:c697933-697655 [Serratia plymuthica]
ATGGCTACTGCTACGTCGATAAAAATTGATGATGAGCTTAAGCAACGGGTGCAACACATTGCCGCCACCCAGCAGCGCAG
CGCACACTGGATTATGCGTGAAGCTATTCGTGAATATGTCGAGCGTGAAGAAAAACGCGAGGCTGTTCGCAACGATGCAT
TGCAGGCATGGGAAACCTATCAGGCCAATGGTAAACATCTGACTCAGGACGAGGCTGACGGCTGGCTCGCCGATCTGGAA
AAAGGTGATGATACGGAGATACCCGGGTGCCACAACTGA

Structures

Antitoxin

• AlphaFold DB: A0A829GEF2