Detailed information of TA system TA155265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2448276..2448802
• Replicon: chromosome
• Accession: NZ_CP051751
• Organism: Escherichia coli strain SCU-301

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: HHJ43_RS11590
• Protein ID: WP_000323025.1
• Coordinates: 2448515..2448802 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: HHJ43_RS11585
• Protein ID: WP_000534858.1
• Coordinates: 2448276..2448515 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ43_RS11560	2443538..2444152	+	615	WP_001265627.1	hypothetical protein	-
HHJ43_RS11565	2444149..2445177	+	1029	WP_001345551.1	DUF4868 domain-containing protein	-
HHJ43_RS11570	2445661..2446974	+	1314	WP_089591175.1	ISNCY family transposase	-
HHJ43_RS11575	2447411..2447743	-	333	WP_001301033.1	protein FlxA	-
HHJ43_RS11580	2447946..2448251	-	306	WP_001326990.1	hypothetical protein	-
HHJ43_RS11585	2448276..2448515	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
HHJ43_RS11590	2448515..2448802	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
HHJ43_RS11595	2448874..2449029	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
HHJ43_RS11600	2449246..2449497	+	252	WP_000980994.1	hypothetical protein	-
HHJ43_RS11605	2449564..2449842	+	279	WP_012304870.1	hypothetical protein	-
HHJ43_RS11610	2449844..2450893	+	1050	WP_001265199.1	DUF968 domain-containing protein	-
HHJ43_RS11615	2450907..2451659	+	753	WP_001047135.1	antitermination protein	-
HHJ43_RS11620	2451937..2452026	-	90	WP_120795389.1	hypothetical protein	-
HHJ43_RS11625	2452081..2452293	-	213	WP_000087756.1	cold shock-like protein CspF	-
HHJ43_RS11630	2452594..2452809	+	216	WP_000066484.1	cold shock-like protein CspB	-
HHJ43_RS11635	2453563..2453778	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2417005..2497017	80012

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T155265 WP_000323025.1 NZ_CP051751:2448515-2448802 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T155265 NZ_CP068761:c891464-891045 [Neisseria gonorrhoeae]
ATGATTTTGCTGGACACGAATGTGATTTCCGAACCTTTGCGCCCACAACCCAATGAACGTGTGGTGGCATGGTTGGATAG
TTTGATATTGGAAGATGTGTATTTGTCTGCCATTACTGTTGCAGAATTGCGTTTGGGTGTGGCGTTGTTGCTCAATGGCA
AGAAAAAGAATGTGCTGCACGAACGTTTGGAACAATCCATTTTGCCTTTATTTGCGGGGCGGATTCTGCCTTTTGATGAA
CCGGTTGCCGCAATCTATGCGCAAATTCGTTCCTATGCCAAAACACATGGCAAAGAGATTGCTGCCGCAGACGGCTATAT
TGCCGCCACTGCAAAACAGCACAGTTTGACAGTTGCTACGCGTGATACCGGCTCATTTTTTGCGGCCGATGTCGCGGTGT
TCAATCCGTGGCACGATTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT155265 WP_000534858.1 NZ_CP051751:2448276-2448515 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT155265 NZ_CP068761:c891700-891464 [Neisseria gonorrhoeae]
ATGGCTTCTGTTGTGATTAGAAATTTATCCGAGGCCACGCACAACGCAATCAAATTCCGTGCGCGAGCCGCAGGGCGCAG
TACCGAAGCAGAAATCCGCTTAATTTTGGATAACATCGCCAAAGCACAACAAACTGTACGTTTGGGGTCAATGTTGGCAT
CAATAGGGCAGGAAATCGGAGGTGTTGAGCTGGAAGACGTACGCGGTCGTAATACTGATAACGAGGTTTCTTTGTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A4V1CTS8