Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 999019..999673 Replicon chromosome
Accession NZ_CP051751
Organism Escherichia coli strain SCU-301

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HHJ43_RS04715 Protein ID WP_000244781.1
Coordinates 999266..999673 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HHJ43_RS04710 Protein ID WP_000354046.1
Coordinates 999019..999285 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ43_RS04690 995108..996541 - 1434 WP_024175772.1 6-phospho-beta-glucosidase BglA -
HHJ43_RS04695 996586..996897 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
HHJ43_RS04700 997061..997720 + 660 WP_000250269.1 hemolysin III family protein -
HHJ43_RS04705 997797..998777 - 981 WP_096986668.1 tRNA-modifying protein YgfZ -
HHJ43_RS04710 999019..999285 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HHJ43_RS04715 999266..999673 + 408 WP_000244781.1 protein YgfX Toxin
HHJ43_RS04720 999713..1000234 - 522 WP_001055874.1 flavodoxin FldB -
HHJ43_RS04725 1000346..1001242 + 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
HHJ43_RS04730 1001267..1001977 + 711 WP_000715222.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HHJ43_RS04735 1001983..1003716 + 1734 WP_096986669.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T155255 WP_000244781.1 NZ_CP051751:999266-999673 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T155255 NZ_CP068717:239263-239613 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT155255 WP_000354046.1 NZ_CP051751:999019-999285 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT155255 NZ_CP068717:238971-239258 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References