Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4583758..4584255 Replicon chromosome
Accession NZ_CP051744
Organism Escherichia coli strain SCU-484

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HHJ53_RS21915 Protein ID WP_001173343.1
Coordinates 4583758..4583931 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag HHJ53_RS21920 Protein ID WP_001312331.1
Coordinates 4583959..4584255 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ53_RS21905 4580428..4581885 + 1458 WP_000856825.1 dipeptide/tripeptide permease DtpC -
HHJ53_RS21910 4582122..4583639 + 1518 WP_000003805.1 lysine--tRNA ligase -
HHJ53_RS21915 4583758..4583931 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HHJ53_RS21920 4583959..4584255 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HHJ53_RS21925 4584484..4584756 - 273 WP_000405647.1 N-acetyltransferase -
HHJ53_RS21930 4584768..4584998 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
HHJ53_RS21935 4585179..4586810 + 1632 WP_001216466.1 sensor histidine kinase -
HHJ53_RS21940 4586807..4587526 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4560540..4584255 23715


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T155244 WP_001173343.1 NZ_CP051744:c4583931-4583758 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T155244 NZ_CP068708:c1007675-1007409 [Streptococcus suis]
ATGAAGACCAGTTATAAACTCGTACCAACCGGTCGTTTTATCAAACAGTTGAAAAAGCTGGACAAATTTACTCAAAAGCA
GATTACCAACTACCTGTCTAGTCATGTGACCGACAACCCTAGACAATACGGAAAAGCCTTGACTGCTAATCGTTCTGGCC
AATGGCGCTATCGTATCGGCAATTACAGGGTTATCGTCAGTATTGAAGATGACAAATTGATTGTAACGGCCCTTGAAGTC
GGGCATCGGAAAGAAATATATAAATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT155244 WP_001312331.1 NZ_CP051744:c4584255-4583959 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT155244 NZ_CP068708:c1007910-1007659 [Streptococcus suis]
ATGGCAGCTATTACATTGAAAGTTTCTGAGCAGGACAAGCTCTTTATGCAGGCTATGGCAAAGTTTGAAGGTGTGAGTTT
GTCAGAACTCATTCGGACTAAAACCCTTGAAGCACTTGAAGATGAGTACGATGCGCGTGTGGCTGACATTGCACTTGCAG
AATACGAGGCTGACTTGGCGAATGGCATTGAGCCGATAAGCTGGGAGGAAATGTTGGAAGATTTGGGGCTGACGGATGAA
GACCAGTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References