Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4269534..4270110 Replicon chromosome
Accession NZ_CP051744
Organism Escherichia coli strain SCU-484

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag HHJ53_RS20425 Protein ID WP_001350372.1
Coordinates 4269823..4270110 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0G9FLJ7
Locus tag HHJ53_RS20420 Protein ID WP_000063155.1
Coordinates 4269534..4269836 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ53_RS20405 4266062..4268212 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
HHJ53_RS20410 4268330..4268533 + 204 WP_000467859.1 YbdD/YjiX family protein -
HHJ53_RS20415 4268544..4269500 + 957 WP_001350371.1 GTPase -
HHJ53_RS20420 4269534..4269836 - 303 WP_000063155.1 BrnA antitoxin family protein Antitoxin
HHJ53_RS20425 4269823..4270110 - 288 WP_001350372.1 BrnT family toxin Toxin
HHJ53_RS20430 4270307..4271473 + 1167 WP_000800824.1 restriction endonuclease -
HHJ53_RS20435 4271537..4273156 + 1620 WP_001029741.1 type I restriction-modification system subunit M -
HHJ53_RS20440 4273146..4274477 + 1332 WP_000505211.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T155242 WP_001350372.1 NZ_CP051744:c4270110-4269823 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T155242 NZ_CP068708:c777450-776605 [Streptococcus suis]
ATGAATAAAGCTAAGCTAACAGCACTCTGTCATAAAATATCAAAGAATACTGGATTAACCTTTAATTCCGTGATGACCTA
TTACTTTCTAGAAGTGATTTTGAAAAAATTAAGTCAGAGTACCTATTCAAATCACTATATATTCAAAGGAGGATTCCTAC
TATCAAATGTTATTGGAGTTGAATCTCGTAGCACGGTTGATATTGATTTTTTGTTCCATCAAATAACACTCTCAGAAGAT
ACTGTGAAGCAGCAACTCAAGGAAATTTTAGCAGATTCAGAAGAAGGTATTTCTTTTGTGATTCAATCCATCACAGCTAT
TAAAGAAAGTGATGACTATGGTGGCTATCGAGTAACAATTTTGTGCCAACTTGAGAATATTAAACAAGTTATTCATTTAG
ATATTGCGACAGGCGATGTTGTAACTCCTCAGCCGATTACATACGACTATAAAGCTATTTTTGATGAAGATAATTTTCCA
ATCATTGCTTATACAATTGAAACAATTCTAGCTGAGAAACTTCAAACCATCTATTCACGTAACTTCTTGAACAGCAGAAG
CAAAGATTTTTATGATGTTTATATTCTATCAAAACTGAAGAAAGAGGACATTGACTTCATTCAATTGAAAAATGCCTGTC
AGAGAACATTTTCATATCGCGAAACAGAGCTCGATTTTGAAAAGATTATTGAACTACTCGAGAGGTTCAAATCTGACCCT
ATTCAGAATAAACAATGGCAAAATTATTCGAAAAAATATAGCTATACAAAGGTGATTTCACTTGCAGATGTTCTTGATGA
GATGATTAGTCTTATTACAATTATTATTTCTACTGACTCTGATTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11572.05 Da        Isoelectric Point: 9.4646

>AT155242 WP_000063155.1 NZ_CP051744:c4269836-4269534 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT155242 NZ_CP068708:c778037-777447 [Streptococcus suis]
ATGTCAAAAAAAGAGATTCTACTTGAATTTATAGAAAAACACAAAGGCATTATCACCTATAAAGATTGTAAAGCACTAAA
CATTCCAACAATATATTTGACAAGACTAGAAAAAGAAGGAATTCTATACCGCGTCGAAAAAGGAATTTTCCTAACCCAGA
ATGGAGACTACGACGAATACTACTTCTTTCAGTACCGATATCCGAAAGCTGTTTTCTCATATATCTCAGCGCTTTATCTG
CAACAATTTACAGATGAAATTCCTCAATATTTTGACGTAACCATTCCTAGAGGCTACCGTTTTAATACTCCTCCAGCTAA
TCTGAACATTCATTCCGTTTCTAAGGAATATAGCGAACTAGGAATTACTCTTGTAAATACCCCTATGGGAAACGAGGTAA
GAGCGTATGATTTCGAACGTATTATCTGTGATTTTGTGATTCATCGAGAAAAAATAGATACTGAGCTTTTTGTCAAAACA
CTTCAACATTACGGAAACTATCCTAAAAAAAATCTTACAAAACTCTATGAATATGCGACAAAAATGAACACCTTGGCCAA
GGTCAAACAAACTCTGGAGGTCCTAGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0G9FLJ7

References