Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4159072..4159622 Replicon chromosome
Accession NZ_CP051744
Organism Escherichia coli strain SCU-484

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag HHJ53_RS19880 Protein ID WP_001160966.1
Coordinates 4159072..4159386 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HHJ53_RS19885 Protein ID WP_000125566.1
Coordinates 4159389..4159622 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ53_RS19855 4154747..4156033 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
HHJ53_RS19860 4156033..4157022 + 990 WP_000241228.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HHJ53_RS19865 4157019..4157840 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HHJ53_RS19870 4157843..4158220 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HHJ53_RS19875 4158227..4159075 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HHJ53_RS19880 4159072..4159386 - 315 WP_001160966.1 CcdB family protein Toxin
HHJ53_RS19885 4159389..4159622 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HHJ53_RS19890 4159708..4160187 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
HHJ53_RS19895 4160379..4162241 - 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
HHJ53_RS19900 4162234..4162764 - 531 WP_169057904.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HHJ53_RS19905 4162872..4164203 - 1332 WP_001183207.1 MFS transporter -
HHJ53_RS19910 4164262..4164549 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T155239 WP_001160966.1 NZ_CP051744:c4159386-4159072 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T155239 NZ_CP068707:234383-234853 [Escherichia coli]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT155239 WP_000125566.1 NZ_CP051744:c4159622-4159389 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT155239 NZ_CP068707:233943-234386 [Escherichia coli]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References