Detailed information of TA system TA155220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 137859..138394
• Replicon: plasmid pSCU-105-1
• Accession: NZ_CP051739
• Organism: Escherichia coli strain SCU-105

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HHJ23_RS25510
• Protein ID: WP_169063301.1
• Coordinates: 137859..138146 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HHJ23_RS25515
• Protein ID: WP_169063302.1
• Coordinates: 138143..138394 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ23_RS25490	134576..135061	-	486	WP_169063297.1	LysR family transcriptional regulator	-
HHJ23_RS25495	135156..135665	+	510	WP_169063298.1	phenolic acid decarboxylase	-
HHJ23_RS25500	135678..136724	+	1047	WP_169063299.1	alpha/beta hydrolase	-
HHJ23_RS25505	136841..137416	-	576	WP_169063300.1	proQ/FINO family protein	-
HHJ23_RS25510	137859..138146	-	288	WP_169063301.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HHJ23_RS25515	138143..138394	-	252	WP_169063302.1	antitoxin	Antitoxin
HHJ23_RS25520	139351..140208	-	858	WP_169063303.1	incFII family plasmid replication initiator RepA	-
HHJ23_RS25525	140201..140275	-	75	WP_001375168.1	RepA leader peptide Tap	-
HHJ23_RS25530	140521..140769	-	249	WP_000083839.1	replication regulatory protein RepA	-
HHJ23_RS25535	141009..141599	-	591	WP_000766807.1	DUF2726 domain-containing protein	-
HHJ23_RS25540	141637..141846	-	210	WP_001333231.1	hemolysin expression modulator Hha	-
HHJ23_RS25545	141892..142353	-	462	WP_126755969.1	thermonuclease family protein	-
HHJ23_RS25550	142598..142810	-	213	WP_001301409.1	ANR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id	faeJ / faeI / faeH / faeF / faeE / faeD / faeC	1..172627	172627
	-	inside	IScluster/Tn	-	-	125179..134500	9321

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11210.29 Da
• Isoelectric Point: 10.5833

>T155220 WP_169063301.1 NZ_CP051739:c138146-137859 [Escherichia coli]
MTYTVKFRDDALKEWFRLDKAIQQQFAKKLKKCSENPHIAFAKLRGLKDCYKIKLRTSGFRLVYQVIDDMLIIAVVAVGK
RERSNVYHLASERMK

Nucleotide

• Length: 288 bp

>T155220 NZ_CP068706:c1708007-1707633 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9189.42 Da
• Isoelectric Point: 4.0927

>AT155220 WP_169063302.1 NZ_CP051739:c138394-138143 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDAMDDQELVKLVTERSSQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT155220 NZ_CP068706:c1708464-1708096 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA