Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 49308..50083 Replicon plasmid pSCU-105-1
Accession NZ_CP051739
Organism Escherichia coli strain SCU-105

Toxin (Protein)


Gene name tacT Uniprot ID A0A6D0V6Q2
Locus tag HHJ23_RS24950 Protein ID WP_000405252.1
Coordinates 49308..49790 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID -
Locus tag HHJ23_RS24955 Protein ID WP_001346256.1
Coordinates 49781..50083 (-) Length 101 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ23_RS24920 45002..45793 - 792 Protein_55 incFII family plasmid replication initiator RepA -
HHJ23_RS24925 45987..47528 + 1542 WP_001298859.1 IS21-like element ISEc12 family transposase -
HHJ23_RS24930 47543..48289 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
HHJ23_RS24935 48371..48445 - 75 Protein_58 replication protein RepA -
HHJ23_RS24940 48438..48512 - 75 WP_001365705.1 RepA leader peptide Tap -
HHJ23_RS24945 48750..49004 - 255 WP_000083846.1 replication regulatory protein RepA -
HHJ23_RS24950 49308..49790 - 483 WP_000405252.1 GNAT family N-acetyltransferase Toxin
HHJ23_RS24955 49781..50083 - 303 WP_001346256.1 DUF1778 domain-containing protein Antitoxin
HHJ23_RS24960 50292..50553 - 262 Protein_63 type I toxin-antitoxin system Hok family toxin -
- 50606..50657 + 52 NuclAT_1 - -
- 50606..50657 + 52 NuclAT_1 - -
- 50606..50657 + 52 NuclAT_1 - -
- 50606..50657 + 52 NuclAT_1 - -
HHJ23_RS24965 50756..51003 - 248 Protein_64 DUF2726 domain-containing protein -
HHJ23_RS24970 51492..51704 - 213 WP_049035740.1 ANR family transcriptional regulator -
HHJ23_RS24975 51835..52395 - 561 WP_169063274.1 fertility inhibition protein FinO -
HHJ23_RS24980 52450..53196 - 747 WP_000205718.1 conjugal transfer pilus acetylase TraX -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id faeJ / faeI / faeH / faeF / faeE / faeD / faeC 1..172627 172627


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(31-149)

Antitoxin

(15-99)


Sequences


Toxin        


Download         Length: 161 a.a.        Molecular weight: 17687.47 Da        Isoelectric Point: 7.1036

>T155214 WP_000405252.1 NZ_CP051739:c49790-49308 [Escherichia coli]
MEINVTAPVLLTDEHILQPFDCGNEVLSNWLHGRAMKNQMLNASRTFVICLEGTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T155214 NZ_CP068706:742199-742606 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11602.19 Da        Isoelectric Point: 4.4569

>AT155214 WP_001346256.1 NZ_CP051739:c50083-49781 [Escherichia coli]
MEVVMPTTASIRKTPRENQINIRATDEERAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDDECYDSFITQLE
APVQNAEGRERLMAVKPEWK

Download         Length: 303 bp

>AT155214 NZ_CP068706:741952-742218 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A6D0V6Q2


Antitoxin

Source ID Structure

References