Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 9741..10384 Replicon plasmid pSCU-105-1
Accession NZ_CP051739
Organism Escherichia coli strain SCU-105

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag HHJ23_RS24715 Protein ID WP_001044768.1
Coordinates 9968..10384 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag HHJ23_RS24710 Protein ID WP_001261287.1
Coordinates 9741..9971 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ23_RS24690 4902..5990 + 1089 WP_049036913.1 transcriptional repressor PifC -
HHJ23_RS24695 5992..8217 + 2226 WP_049036910.1 exclusion suppressor FxsA -
HHJ23_RS24700 8267..9166 - 900 WP_000963206.1 nucleotide-binding protein -
HHJ23_RS24705 9156..9446 - 291 WP_000111771.1 hypothetical protein -
HHJ23_RS24710 9741..9971 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HHJ23_RS24715 9968..10384 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
HHJ23_RS24720 10546..12684 - 2139 WP_000350635.1 AAA family ATPase -
HHJ23_RS24725 13002..13253 + 252 WP_049036907.1 hypothetical protein -
HHJ23_RS24730 13253..13843 + 591 WP_024234847.1 hypothetical protein -
HHJ23_RS24735 13861..14208 - 348 WP_024234846.1 hypothetical protein -
HHJ23_RS24740 14337..14672 + 336 WP_024234845.1 colicin transporter -
HHJ23_RS24745 14690..15001 - 312 WP_169063268.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id faeJ / faeI / faeH / faeF / faeE / faeD / faeC 1..172627 172627


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T155213 WP_001044768.1 NZ_CP051739:9968-10384 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T155213 NZ_CP068706:614478-614774 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT155213 WP_001261287.1 NZ_CP051739:9741-9971 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT155213 NZ_CP068706:614776-615171 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References