Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3803..4328 Replicon plasmid pSCU-105-1
Accession NZ_CP051739
Organism Escherichia coli strain SCU-105

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag HHJ23_RS24680 Protein ID WP_001159871.1
Coordinates 3803..4108 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag HHJ23_RS24685 Protein ID WP_000813634.1
Coordinates 4110..4328 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ23_RS24650 1..340 + 340 WP_169063269.1 DUF4113 domain-containing protein -
HHJ23_RS24655 345..737 - 393 WP_000340833.1 plasmid partitioning/stability family protein -
HHJ23_RS24660 742..1713 - 972 WP_049036915.1 plasmid segregation protein parM -
HHJ23_RS24665 1942..2586 + 645 WP_000633911.1 AAA family ATPase -
HHJ23_RS24670 2580..2855 + 276 WP_000239529.1 hypothetical protein -
HHJ23_RS24675 2993..3802 - 810 WP_000016979.1 site-specific integrase -
HHJ23_RS24680 3803..4108 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
HHJ23_RS24685 4110..4328 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HHJ23_RS24690 4902..5990 + 1089 WP_049036913.1 transcriptional repressor PifC -
HHJ23_RS24695 5992..8217 + 2226 WP_049036910.1 exclusion suppressor FxsA -
HHJ23_RS24700 8267..9166 - 900 WP_000963206.1 nucleotide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id faeJ / faeI / faeH / faeF / faeE / faeD / faeC 1..172627 172627


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T155212 WP_001159871.1 NZ_CP051739:c4108-3803 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T155212 NZ_CP068706:548567-548881 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT155212 WP_000813634.1 NZ_CP051739:c4328-4110 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT155212 NZ_CP068706:548878-549294 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References