Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2958579..2959363 Replicon chromosome
Accession NZ_CP051738
Organism Escherichia coli strain SCU-105

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag HHJ23_RS14355 Protein ID WP_000613626.1
Coordinates 2958869..2959363 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag HHJ23_RS14350 Protein ID WP_001110447.1
Coordinates 2958579..2958872 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ23_RS14340 2953729..2954688 - 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
HHJ23_RS14345 2955261..2958446 + 3186 WP_000827427.1 ribonuclease E -
HHJ23_RS14350 2958579..2958872 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
HHJ23_RS14355 2958869..2959363 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
HHJ23_RS14360 2959458..2960411 - 954 WP_001212762.1 flagellar hook-associated protein FlgL -
HHJ23_RS14365 2960423..2962066 - 1644 WP_001544507.1 flagellar hook-associated protein FlgK -
HHJ23_RS14370 2962132..2963073 - 942 WP_001544506.1 flagellar assembly peptidoglycan hydrolase FlgJ -
HHJ23_RS14375 2963073..2964170 - 1098 WP_000589326.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T155202 WP_000613626.1 NZ_CP051738:2958869-2959363 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T155202 NZ_CP068696:2583647-2583750 [Salmonella enterica subsp. enterica serovar Typhimurium]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT155202 WP_001110447.1 NZ_CP051738:2958579-2958872 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT155202 NZ_CP068696:c2583752-2583607 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References