Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3638836..3639412 Replicon chromosome
Accession NZ_CP051735
Organism Escherichia coli strain SCU-108

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HHJ26_RS17390 Protein ID WP_001297643.1
Coordinates 3638836..3639123 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag HHJ26_RS17395 Protein ID WP_000063156.1
Coordinates 3639110..3639412 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ26_RS17370 3635089..3636369 - 1281 WP_001037382.1 DUF445 domain-containing protein -
HHJ26_RS17375 3636410..3637642 - 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
HHJ26_RS17380 3637788..3637883 - 96 Protein_3391 dienelactone hydrolase family protein -
HHJ26_RS17385 3637940..3638637 + 698 Protein_3392 IS1 family transposase -
HHJ26_RS17390 3638836..3639123 + 288 WP_001297643.1 BrnT family toxin Toxin
HHJ26_RS17395 3639110..3639412 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
HHJ26_RS17400 3639446..3640402 - 957 WP_001297640.1 GTPase -
HHJ26_RS17405 3640413..3640616 - 204 WP_000467859.1 YbdD/YjiX family protein -
HHJ26_RS17410 3640666..3642816 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T155170 WP_001297643.1 NZ_CP051735:3638836-3639123 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T155170 NZ_CP068687:c35067-34651 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT155170 WP_000063156.1 NZ_CP051735:3639110-3639412 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT155170 NZ_CP068687:c35294-35064 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References