Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1675543..1676126 Replicon chromosome
Accession NZ_CP051735
Organism Escherichia coli strain SCU-108

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag HHJ26_RS08070 Protein ID WP_000254750.1
Coordinates 1675543..1675878 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag HHJ26_RS08075 Protein ID WP_000581937.1
Coordinates 1675878..1676126 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ26_RS08055 1671429..1672727 - 1299 WP_000036723.1 phosphopyruvate hydratase -
HHJ26_RS08060 1672815..1674452 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
HHJ26_RS08065 1674680..1675471 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
HHJ26_RS08070 1675543..1675878 - 336 WP_000254750.1 endoribonuclease MazF Toxin
HHJ26_RS08075 1675878..1676126 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
HHJ26_RS08080 1676204..1678438 - 2235 WP_000226815.1 GTP pyrophosphokinase -
HHJ26_RS08085 1678486..1679787 - 1302 WP_000046816.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T155162 WP_000254750.1 NZ_CP051735:c1675878-1675543 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T155162 NZ_CP068685:c105011-104727 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT155162 WP_000581937.1 NZ_CP051735:c1676126-1675878 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT155162 NZ_CP068685:c105249-105001 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References