Detailed information of TA system TA155147

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3955489..3956284
• Replicon: chromosome
• Accession: NZ_CP051733
• Organism: Escherichia coli strain SCU-109

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A658LKE6
• Locus tag: HHJ27_RS18675
• Protein ID: WP_001596171.1
• Coordinates: 3955910..3956284 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HHJ27_RS18670
• Protein ID: WP_001596170.1
• Coordinates: 3955489..3955863 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ27_RS18635	3951873..3952106	+	234	WP_001117568.1	DUF905 family protein	-
HHJ27_RS18640	3952230..3952712	+	483	Protein_3632	DUF932 domain-containing protein	-
HHJ27_RS18645	3952804..3952866	+	63	Protein_3633	antirestriction protein	-
HHJ27_RS18650	3952933..3954095	+	1163	WP_085947598.1	IS3-like element IS3 family transposase	-
HHJ27_RS18655	3954098..3954550	+	453	WP_096035156.1	antirestriction protein	-
HHJ27_RS18660	3954566..3955042	+	477	WP_001186726.1	RadC family protein	-
HHJ27_RS18665	3955105..3955326	+	222	WP_000692323.1	DUF987 domain-containing protein	-
HHJ27_RS18670	3955489..3955863	+	375	WP_001596170.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HHJ27_RS18675	3955910..3956284	+	375	WP_001596171.1	TA system toxin CbtA family protein	Toxin
HHJ27_RS18680	3956281..3956772	+	492	WP_001596172.1	hypothetical protein	-
HHJ27_RS18685	3956784..3956981	+	198	WP_001596173.1	DUF957 domain-containing protein	-
HHJ27_RS18690	3957066..3957908	+	843	Protein_3642	DUF4942 domain-containing protein	-
HHJ27_RS18695	3958250..3958420	+	171	Protein_3643	IS110 family transposase	-
HHJ27_RS18700	3959231..3960214	+	984	WP_001596176.1	KpsF/GutQ family sugar-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3952933..3958420	5487

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14161.14 Da
• Isoelectric Point: 6.7041

>T155147 WP_001596171.1 NZ_CP051733:3955910-3956284 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLARLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYELVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T155147 NZ_CP068682:1115443-1115652 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAAACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13813.62 Da
• Isoelectric Point: 6.7387

>AT155147 WP_001596170.1 NZ_CP051733:3955489-3955863 [Escherichia coli]
VSDTLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLTVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT155147 NZ_CP068682:1115138-1115431 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A658LKE6