Detailed information of TA system TA155134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2331538..2333126
• Replicon: chromosome
• Accession: NZ_CP051733
• Organism: Escherichia coli strain SCU-109

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HHJ27_RS11040
• Protein ID: WP_001595766.1
• Coordinates: 2331538..2332860 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HHJ27_RS11045
• Protein ID: WP_001296726.1
• Coordinates: 2332860..2333126 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ27_RS11000	2326572..2327144	-	573	WP_000789913.1	NAD(P)H-dependent oxidoreductase	-
HHJ27_RS11005	2327295..2327486	-	192	WP_001469139.1	amidohydrolase family protein	-
HHJ27_RS11010	2327437..2327856	-	420	WP_001322416.1	amidohydrolase family protein	-
HHJ27_RS11015	2327816..2327971	-	156	WP_001322415.1	hypothetical protein	-
HHJ27_RS11020	2328074..2329021	-	948	WP_000854796.1	aldo/keto reductase	-
HHJ27_RS11025	2329095..2329784	-	690	WP_000801313.1	type 1 glutamine amidotransferase domain-containing protein	-
HHJ27_RS11030	2329793..2330101	-	309	WP_001221046.1	hypothetical protein	-
HHJ27_RS11035	2330241..2331140	+	900	WP_001057776.1	LysR family transcriptional regulator	-
HHJ27_RS11040	2331538..2332860	-	1323	WP_001595766.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HHJ27_RS11045	2332860..2333126	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HHJ27_RS11050	2333310..2334710	-	1401	WP_169057745.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49370.02 Da
• Isoelectric Point: 8.5830

>T155134 WP_001595766.1 NZ_CP051733:c2332860-2331538 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK

Nucleotide

• Length: 1323 bp

>T155134 NZ_CP068680:2119873-2120208 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGATCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT155134 WP_001296726.1 NZ_CP051733:c2333126-2332860 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT155134 NZ_CP068680:2119625-2119873 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA