Detailed information of TA system TA155123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 698301..698725
• Replicon: chromosome
• Accession: NZ_CP051733
• Organism: Escherichia coli strain SCU-109

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: HHJ27_RS03345
• Protein ID: WP_001586653.1
• Coordinates: 698537..698725 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: HHJ27_RS03340
• Protein ID: WP_000125566.1
• Coordinates: 698301..698534 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ27_RS03315	693374..693661	+	288	WP_001596509.1	ferredoxin-like protein FixX	-
HHJ27_RS03320	693720..695051	+	1332	WP_001183198.1	MFS transporter	-
HHJ27_RS03325	695159..695689	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
HHJ27_RS03330	695682..697544	+	1863	WP_000377117.1	glutathione-regulated potassium-efflux system protein KefC	-
HHJ27_RS03335	697736..698215	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
HHJ27_RS03340	698301..698534	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HHJ27_RS03345	698537..698725	+	189	WP_001586653.1	CcdB family protein	Toxin
HHJ27_RS03350	698722..698838	+	117	WP_001389634.1	CcdB family protein	-
HHJ27_RS03355	698835..699683	-	849	WP_000257195.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
HHJ27_RS03360	699690..700067	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
HHJ27_RS03365	700070..700891	-	822	WP_001065361.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
HHJ27_RS03370	700888..701877	-	990	WP_000241280.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
HHJ27_RS03375	701877..703163	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-57)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7029.22 Da
• Isoelectric Point: 8.7938

>T155123 WP_001586653.1 NZ_CP051733:698537-698725 [Escherichia coli]
MQFTVYRSRGSNAAFPFVIDVTSDIIGEINRSIVIPLTPIERFIRIRPPERLNTILLLVDGS

Nucleotide

• Length: 189 bp

>T155123 NZ_CP068680:c399711-399424 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT155123 WP_000125566.1 NZ_CP051733:698301-698534 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT155123 NZ_CP068680:c399437-399135 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9