Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 571698..572274 Replicon chromosome
Accession NZ_CP051733
Organism Escherichia coli strain SCU-109

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag HHJ27_RS02725 Protein ID WP_001131965.1
Coordinates 571698..571985 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2S5UGC7
Locus tag HHJ27_RS02730 Protein ID WP_019842999.1
Coordinates 571972..572274 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ27_RS02710 567238..568662 - 1425 WP_000535015.1 restriction endonuclease subunit S -
HHJ27_RS02715 568652..570271 - 1620 WP_001029743.1 type I restriction-modification system subunit M -
HHJ27_RS02720 570335..571501 - 1167 WP_000800825.1 restriction endonuclease -
HHJ27_RS02725 571698..571985 + 288 WP_001131965.1 BrnT family toxin Toxin
HHJ27_RS02730 571972..572274 + 303 WP_019842999.1 BrnA antitoxin family protein Antitoxin
HHJ27_RS02735 572308..573264 - 957 WP_001295525.1 GTPase -
HHJ27_RS02740 573275..573478 - 204 WP_000467859.1 YbdD/YjiX family protein -
HHJ27_RS02745 573528..575678 - 2151 WP_001439331.1 pyruvate/proton symporter BtsT -
HHJ27_RS02750 576068..576580 - 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T155122 WP_001131965.1 NZ_CP051733:571698-571985 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T155122 NZ_CP068680:72268-72546 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAA
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.00 Da        Isoelectric Point: 9.8223

>AT155122 WP_019842999.1 NZ_CP051733:571972-572274 [Escherichia coli]
MSMVKHKRGTSSTLNVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT155122 NZ_CP068680:72005-72265 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2S5UGC7

References