Detailed information of TA system TA155115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 55902..56527
• Replicon: plasmid pSCU-111-2
• Accession: NZ_CP051729
• Organism: Escherichia coli strain SCU-111

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HHJ28_RS24070
• Protein ID: WP_097336710.1
• Coordinates: 55902..56300 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8H9EG71
• Locus tag: HHJ28_RS24075
• Protein ID: WP_000450526.1
• Coordinates: 56300..56527 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ28_RS24050	51009..51572	+	564	WP_096037668.1	class I SAM-dependent methyltransferase	-
HHJ28_RS24055	51716..52498	-	783	WP_097748551.1	IS21-like element IS100kyp family helper ATPase IstB	-
HHJ28_RS24060	52495..53517	-	1023	WP_000255956.1	IS21-like element IS100 family transposase	-
HHJ28_RS24065	53641..55893	+	2253	WP_169062163.1	type IV conjugative transfer system coupling protein TraD	-
HHJ28_RS24070	55902..56300	-	399	WP_097336710.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HHJ28_RS24075	56300..56527	-	228	WP_000450526.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..66467	66467

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14945.21 Da
• Isoelectric Point: 7.8605

>T155115 WP_097336710.1 NZ_CP051729:c56300-55902 [Escherichia coli]
MLKFMLDTNICIFTIKNKPTSVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T155115 NZ_CP068678:3492113-3492412 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.53 Da
• Isoelectric Point: 4.5358

>AT155115 WP_000450526.1 NZ_CP051729:c56527-56300 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGESWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT155115 NZ_CP068678:3492409-3492699 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCGACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGCGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA