Detailed information of TA system TA155114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 28423..28970
• Replicon: plasmid pSCU-111-2
• Accession: NZ_CP051729
• Organism: Escherichia coli strain SCU-111

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: HHJ28_RS23900
• Protein ID: WP_169062160.1
• Coordinates: 28689..28970 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: F4V9S6
• Locus tag: HHJ28_RS23895
• Protein ID: WP_000079931.1
• Coordinates: 28423..28692 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ28_RS23870	23837..24562	-	726	WP_169062156.1	microcin B17 immunity protein McbE	-
HHJ28_RS23875	24572..25762	-	1191	WP_169062157.1	YcaO-like family protein	-
HHJ28_RS23880	25743..26561	-	819	WP_169062158.1	SagB/ThcOx family dehydrogenase	-
HHJ28_RS23885	26563..27450	-	888	WP_169062159.1	McbB family protein	-
HHJ28_RS23890	27477..27677	-	201	WP_000420495.1	microcin B17	-
HHJ28_RS23895	28423..28692	+	270	WP_000079931.1	hypothetical protein	Antitoxin
HHJ28_RS23900	28689..28970	+	282	WP_169062160.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HHJ28_RS23905	29055..29402	+	348	WP_000142451.1	hypothetical protein	-
HHJ28_RS23910	29422..30012	-	591	WP_042077527.1	hypothetical protein	-
HHJ28_RS23915	30012..30269	-	258	WP_169062161.1	hypothetical protein	-
HHJ28_RS23920	30598..31050	+	453	WP_000670961.1	N-acetyltransferase	-
HHJ28_RS23925	31083..32615	-	1533	WP_001017348.1	NAD(P)-binding domain-containing protein	-
HHJ28_RS23930	32612..32881	-	270	WP_000379710.1	SemiSWEET transporter	-
HHJ28_RS23935	32883..33899	-	1017	WP_016230896.1	Gfo/Idh/MocA family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..66467	66467

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11022.73 Da
• Isoelectric Point: 8.4615

>T155114 WP_169062160.1 NZ_CP051729:28689-28970 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAWQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T155114 NZ_CP068678:3103334-3103699 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10194.55 Da
• Isoelectric Point: 8.8735

>AT155114 WP_000079931.1 NZ_CP051729:28423-28692 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT155114 NZ_CP068678:3103692-3104015 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGTCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9DLR5