Detailed information of TA system TA155109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 103579..104204
• Replicon: plasmid pSCU-111-1
• Accession: NZ_CP051728
• Organism: Escherichia coli strain SCU-111

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HHJ28_RS23305
• Protein ID: WP_097336710.1
• Coordinates: 103806..104204 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8H9EG71
• Locus tag: HHJ28_RS23300
• Protein ID: WP_000450526.1
• Coordinates: 103579..103806 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ28_RS23300	103579..103806	+	228	WP_000450526.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HHJ28_RS23305	103806..104204	+	399	WP_097336710.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HHJ28_RS23310	104213..106411	-	2199	WP_169062101.1	type IV conjugative transfer system coupling protein TraD	-
HHJ28_RS23315	106462..107199	-	738	WP_169062102.1	hypothetical protein	-
HHJ28_RS23320	107402..108133	-	732	WP_169062144.1	complement resistance protein TraT	-
HHJ28_RS23325	108173..108676	-	504	WP_169062103.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	acrB / pet / etpB / etpB / cdtC / cdtB / cdtA / etpA / etpB	1..189717	189717

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14945.21 Da
• Isoelectric Point: 7.8605

>T155109 WP_097336710.1 NZ_CP051728:103806-104204 [Escherichia coli]
MLKFMLDTNICIFTIKNKPTSVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T155109 NZ_CP068651:22498-22752 [Vibrio parahaemolyticus]
ATGAGAATCGTATCTTTTACTGAAGCTAGAAATGGTCTTAAAGCTGTTTTAGACGGTGTAGTTAATGACGCTGATACAAC
AGTTATTACACGCCGTGATTCTGAAGATGCCGTGGTTATGTCTTTAGACTACTACAATAGCCTTATGGAGACAGTTCACT
TACTACGCTCTCCTCAAAACGCTGAACATCTAAATCGCTCGATTGCACAGTACCGTGCTGGTAAAACAACAGCACGAGAG
TTAATCGATGAGTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.53 Da
• Isoelectric Point: 4.5358

>AT155109 WP_000450526.1 NZ_CP051728:103579-103806 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGESWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT155109 NZ_CP068651:22745-23014 [Vibrio parahaemolyticus]
ATGAGTAGTAGTCAACGTTTACTGTCGTGGACTGATGACGCTTGGGATGACTACCTGTATTGGCAAACTCAAGACAAGAA
AACACTCAAGCGCATCAACAAACTCATCAATGATATTAAGCGTTCCTCATTTGAGGGCATCGGTAAACCAGAGCCATTAA
AAGAGAACTTATCTGGCTTTTGGTCTCGCCGTATTGATGACACTAATAGGCTTGTTTACGCAGTCGATGACCAAGCTATA
ACGATAATTTCGTGTCGTTATCACTACTAA