Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4019180..4019756 Replicon chromosome
Accession NZ_CP051727
Organism Escherichia coli strain SCU-111

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag HHJ28_RS19175 Protein ID WP_001350372.1
Coordinates 4019469..4019756 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0G9FLJ7
Locus tag HHJ28_RS19170 Protein ID WP_000063155.1
Coordinates 4019180..4019482 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ28_RS19155 4015708..4017858 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
HHJ28_RS19160 4017976..4018179 + 204 WP_000467859.1 YbdD/YjiX family protein -
HHJ28_RS19165 4018190..4019146 + 957 WP_001350371.1 GTPase -
HHJ28_RS19170 4019180..4019482 - 303 WP_000063155.1 BrnA antitoxin family protein Antitoxin
HHJ28_RS19175 4019469..4019756 - 288 WP_001350372.1 BrnT family toxin Toxin
HHJ28_RS19180 4019953..4021119 + 1167 WP_000800824.1 restriction endonuclease -
HHJ28_RS19185 4021183..4022802 + 1620 WP_001029741.1 type I restriction-modification system subunit M -
HHJ28_RS19190 4022792..4024123 + 1332 WP_000505211.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T155102 WP_001350372.1 NZ_CP051727:c4019756-4019469 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T155102 NZ_CP068641:1532173-1532460 [Vibrio parahaemolyticus]
ATGAAAGTAGTCTGGTCTCCATTAGCACTTCAGAAACTAGGTGATGCAGCGGAGTTTATTTCTTTGGATAATCCACCTGC
GGCAGAGAGGTGGGTAAACGAAGTTTTTGATAAAACTGAACTCCTTGGAAGTATGCCTGAAATGGGGCGGTTTGTTCCTG
AAATGCCCCATACGAACTATCGAGAAATCATCTTTGGCCACTACCGTATTATCTACAGTTTAAGCCACGAAATTCGCATA
CTAACAGTTCGTAACTGTCGTCAAATTTTATCTGAAAGTGACGTGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11572.05 Da        Isoelectric Point: 9.4646

>AT155102 WP_000063155.1 NZ_CP051727:c4019482-4019180 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT155102 NZ_CP068641:1531895-1532176 [Vibrio parahaemolyticus]
ATGAGTCGTATTCATTTCGATCAAGATATTCAGCCTTTGTCTGAGTTTCGTGCCGGTGTAGCTTCATACATCAAACAAAT
CAATGAGACTCGTCGTCCATTGGTTATTACGCAACGTGGCAAAGGTGTTGCTGTTGTTCTCGATGTTGCTGAGTATGAAG
CAATGCAAGAGAAAATCGAATTACTGGAAGAAATGCGCACAGCAGAAGCTCAATTAGCTTCTGGTCTCGGTGTTTCCAAT
GATGATGCGCGTGCTCAAGTACTTGGGCGCATTAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0G9FLJ7

References