Detailed information of TA system TA155095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2808844..2809628
• Replicon: chromosome
• Accession: NZ_CP051727
• Organism: Escherichia coli strain SCU-111

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: HHJ28_RS13520
• Protein ID: WP_000613626.1
• Coordinates: 2809134..2809628 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: HHJ28_RS13515
• Protein ID: WP_001110447.1
• Coordinates: 2808844..2809137 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ28_RS13505	2803994..2804953	-	960	WP_000846326.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
HHJ28_RS13510	2805526..2808711	+	3186	WP_001764425.1	ribonuclease E	-
HHJ28_RS13515	2808844..2809137	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
HHJ28_RS13520	2809134..2809628	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
HHJ28_RS13525	2809723..2810676	-	954	WP_001212764.1	flagellar hook-associated protein FlgL	-
HHJ28_RS13530	2810689..2812332	-	1644	WP_001764424.1	flagellar hook-associated protein FlgK	-
HHJ28_RS13535	2812398..2813339	-	942	WP_001764423.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
HHJ28_RS13540	2813339..2814436	-	1098	WP_001764422.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T155095 WP_000613626.1 NZ_CP051727:2809134-2809628 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T155095 NZ_CP068633:c2084835-2084533 [Vibrio parahaemolyticus]
ATGGCTGAAATAATCTGGACTGAGCCAGCGCTATCCGACCTCAATGATATCGCTGAATACATCGCACTTGAAAATATCGT
AGCTGCAAAACAATTGGTTCAAACGATCTTTTCTAAAGTCGAGCGCTTAGAGGCTTTCCCTGAATCGGGTCGCATTCCAC
CCGAGCTAGAACATTTAAGTTATCGTGAAGTTGTCGTTAATCCATGTCGTGTTTTCTATAAACAAGACGGTGACAAAGTG
TTTATTCTGTTTGTTATGCGTGCAGAGAGAGATTTGCGTAAGTTCCTGTTGAGTAAGCAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT155095 WP_001110447.1 NZ_CP051727:2808844-2809137 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT155095 NZ_CP068633:c2085080-2084823 [Vibrio parahaemolyticus]
ATGAAAGTAGAACTAGTTACATCACTCAAACGTCAAGCAACAAAGATCCTTGCCGATCTCCACGACACTAAAGAACCAGT
GTTAATTACCGAACATGGTAAGCCATCGGCATATCTGGTTGATGTTGATGATTACGAGTTTATGCAAAATCGTTTGGCGA
TTCTGGAGGGGATTGCACGAGGTGAACGTGCATTAGCTGACGGTAAAGTGGTGAGTCACGATGAAGCCAAGGACAAAATG
TCAAAATGGCTGAAATAA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6