Detailed information of TA system TA155084

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1834440..1835271
• Replicon: chromosome
• Accession: NZ_CP051727
• Organism: Escherichia coli strain SCU-111

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: HHJ28_RS08655
• Protein ID: WP_000854814.1
• Coordinates: 1834440..1834814 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: HHJ28_RS08660
• Protein ID: WP_001285585.1
• Coordinates: 1834903..1835271 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ28_RS08615	1829836..1831002	+	1167	WP_024240938.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
HHJ28_RS08620	1831121..1831594	+	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
HHJ28_RS08625	1831792..1832850	+	1059	WP_001200891.1	FUSC family protein	-
HHJ28_RS08630	1833022..1833351	+	330	WP_000450409.1	DUF496 family protein	-
HHJ28_RS08635	1833452..1833634	-	183	WP_001016348.1	hypothetical protein	-
HHJ28_RS08640	1833706..1833834	+	129	Protein_1686	transposase domain-containing protein	-
HHJ28_RS08645	1834123..1834236	-	114	WP_001161660.1	DUF957 domain-containing protein	-
HHJ28_RS08650	1834249..1834443	-	195	WP_000988600.1	hypothetical protein	-
HHJ28_RS08655	1834440..1834814	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HHJ28_RS08660	1834903..1835271	-	369	WP_001285585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
HHJ28_RS08665	1835345..1835566	-	222	WP_000692346.1	DUF987 domain-containing protein	-
HHJ28_RS08670	1835635..1836111	-	477	WP_001186725.1	RadC family protein	-
HHJ28_RS08675	1836127..1836612	-	486	WP_000214415.1	antirestriction protein	-
HHJ28_RS08680	1836704..1837525	-	822	WP_024172605.1	DUF945 domain-containing protein	-
HHJ28_RS08685	1837746..1838156	-	411	WP_000846713.1	hypothetical protein	-
HHJ28_RS08690	1838172..1838855	-	684	WP_000775500.1	hypothetical protein	-
HHJ28_RS08695	1838991..1840061	-	1071	WP_024240939.1	patatin-like phospholipase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T155084 WP_000854814.1 NZ_CP051727:c1834814-1834440 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T155084 NZ_CP068627:c1356728-1356438 [Vibrio parahaemolyticus]
ATGACCTATAAACTCGACTTTAAAAAGAGCGCACTCAAAGAGTGGAAAAAGCTTGGCTCTACTCTGCAACAACAATTTAA
GAAAAAGTTGATAGAACGCTTAGATAACCCACATGTTCCGGCTTCAAAGCTTTCAGGAGCTGACAACATGTATAAAATTA
AGTTGCGTCAATCTGGCTACCGTCTCGTCTACAAAGTTGAAGACGATGTCATCATTGTAACCGTCTTGGCAGTCGGAAAA
CGCGAACGTAGCGATGTTTACCGAAAAGCCATGAAAAGGTTAGATGATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT155084 WP_001285585.1 NZ_CP051727:c1835271-1834903 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT155084 NZ_CP068627:c1356966-1356718 [Vibrio parahaemolyticus]
ATGACCACTAGAATTTTAGCCGATGTTGCTGCAAGCATTACCGAGTTGAAAGCTAACCCTATGAAAGTTGCAACTAGTGC
TTATGGCGAACCTGTTGCTGTATTAAACCGAAATGAGCCAGCATTTTATTGCGTACCTGCCGAAGCGTACGAAATGATGA
TGGACAGACTCGAAGATCTTGAACTACTCGCTATCGCTAAAGAGCGTGAATCTGAAGAGAGCATTTCGGTAAATATTGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A7U9LW60