Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4132450..4133000 Replicon chromosome
Accession NZ_CP051725
Organism Escherichia coli strain SCU-112

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag HHJ29_RS19730 Protein ID WP_001160966.1
Coordinates 4132450..4132764 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HHJ29_RS19735 Protein ID WP_000125566.1
Coordinates 4132767..4133000 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ29_RS19705 4128125..4129411 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
HHJ29_RS19710 4129411..4130400 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HHJ29_RS19715 4130397..4131218 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HHJ29_RS19720 4131221..4131598 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HHJ29_RS19725 4131605..4132453 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HHJ29_RS19730 4132450..4132764 - 315 WP_001160966.1 CcdB family protein Toxin
HHJ29_RS19735 4132767..4133000 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HHJ29_RS19740 4133086..4133565 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
HHJ29_RS19745 4133757..4135619 - 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
HHJ29_RS19750 4135612..4136142 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HHJ29_RS19755 4136250..4137581 - 1332 WP_169063350.1 MFS transporter -
HHJ29_RS19760 4137640..4137927 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T155062 WP_001160966.1 NZ_CP051725:c4132764-4132450 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T155062 NZ_CP068618:351689-352036 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT155062 WP_000125566.1 NZ_CP051725:c4133000-4132767 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT155062 NZ_CP068618:352036-352335 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References