Detailed information of TA system TA155044

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 843168..844002
• Replicon: chromosome
• Accession: NZ_CP051725
• Organism: Escherichia coli strain SCU-112

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: HHJ29_RS04045
• Protein ID: WP_000854690.1
• Coordinates: 843168..843545 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1P7N8
• Locus tag: HHJ29_RS04050
• Protein ID: WP_001305076.1
• Coordinates: 843634..844002 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ29_RS04015	839562..839717	-	156	WP_000729638.1	hypothetical protein	-
HHJ29_RS04020	840149..841082	-	934	Protein_789	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
HHJ29_RS04025	841075..841470	-	396	WP_000208382.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
HHJ29_RS04030	841539..842384	-	846	WP_001529401.1	DUF4942 domain-containing protein	-
HHJ29_RS04035	842469..842666	-	198	WP_000839293.1	DUF957 domain-containing protein	-
HHJ29_RS04040	842683..843171	-	489	WP_000761699.1	hypothetical protein	-
HHJ29_RS04045	843168..843545	-	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
HHJ29_RS04050	843634..844002	-	369	WP_001305076.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HHJ29_RS04055	844052..844696	-	645	WP_000094915.1	hypothetical protein	-
HHJ29_RS04060	844715..844936	-	222	WP_000692312.1	DUF987 domain-containing protein	-
HHJ29_RS04065	844999..845475	-	477	WP_001186727.1	RadC family protein	-
HHJ29_RS04070	845491..845976	-	486	WP_000849565.1	antirestriction protein	-
HHJ29_RS04075	846031..846852	-	822	WP_021536178.1	DUF945 domain-containing protein	-
HHJ29_RS04080	846953..847186	-	234	WP_000902034.1	DUF905 family protein	-
HHJ29_RS04085	847265..847720	-	456	WP_000581502.1	hypothetical protein	-
HHJ29_RS04090	847796..848923	-	1128	Protein_803	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T155044 WP_000854690.1 NZ_CP051725:c843545-843168 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T155044 NZ_CP068610:c4793822-4793538 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13528.33 Da
• Isoelectric Point: 4.7830

>AT155044 WP_001305076.1 NZ_CP051725:c844002-843634 [Escherichia coli]
VSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT155044 NZ_CP068610:c4794054-4793812 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: S1PLF5

Antitoxin

• AlphaFold DB: S1P7N8