Detailed information of TA system TA155042

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 46924..47715
• Replicon: chromosome
• Accession: NZ_CP051725
• Organism: Escherichia coli strain SCU-112

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HHJ29_RS00230
• Protein ID: WP_000691791.1
• Coordinates: 46924..47349 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3L1KVZ6
• Locus tag: HHJ29_RS00235
• Protein ID: WP_000939437.1
• Coordinates: 47380..47715 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ29_RS00205	42590..43087	-	498	WP_169063316.1	hypothetical protein	-
HHJ29_RS00210	43769..44155	-	387	WP_001270938.1	DUF2441 domain-containing protein	-
HHJ29_RS00215	44778..45509	-	732	WP_001216639.1	TIGR02646 family protein	-
HHJ29_RS00220	45506..45727	-	222	WP_125112077.1	hypothetical protein	-
HHJ29_RS00225	45712..46903	-	1192	Protein_44	AAA family ATPase	-
HHJ29_RS00230	46924..47349	-	426	WP_000691791.1	TA system toxin CbtA family protein	Toxin
HHJ29_RS00235	47380..47715	-	336	WP_000939437.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HHJ29_RS00240	47715..48188	-	474	WP_001292742.1	DNA repair protein RadC	-
HHJ29_RS00245	48218..49032	-	815	Protein_48	DUF945 domain-containing protein	-
HHJ29_RS00250	49268..50221	-	954	WP_000290406.1	hypothetical protein	-
HHJ29_RS00255	50814..51428	+	615	WP_000772910.1	inovirus Gp2 family protein	-
HHJ29_RS00260	51546..51764	+	219	WP_000070772.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	44778..68709	23931

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 16490.11 Da
• Isoelectric Point: 10.2376

>T155042 WP_000691791.1 NZ_CP051725:c47349-46924 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVNAVNFLVEKYELVRIDRKGF
SWQEQTPYLRAVDILRARQATGLRIQCKNTNIISRMADSRHDKRLYQRTFKACKRCYYTKT

Nucleotide

• Length: 426 bp

>T155042 NZ_CP068610:4079293-4079511 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12556.56 Da
• Isoelectric Point: 7.6977

>AT155042 WP_000939437.1 NZ_CP051725:c47715-47380 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT155042 NZ_CP068610:4078892-4079266 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A3L1KVZ6