155039

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 33500..34025 Replicon plasmid pSCU-116-2
Accession NZ_CP051721
Organism Escherichia coli strain SCU-116

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag HHJ31_RS24770 Protein ID WP_001159868.1
Coordinates 33500..33805 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag HHJ31_RS24775 Protein ID WP_000813634.1
Coordinates 33807..34025 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ31_RS24755 29410..30576 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
HHJ31_RS24760 31164..31919 - 756 WP_000852146.1 replication initiation protein RepE -
HHJ31_RS24765 32693..33499 - 807 WP_000016982.1 site-specific integrase -
HHJ31_RS24770 33500..33805 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
HHJ31_RS24775 33807..34025 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HHJ31_RS24780 34592..35104 + 513 WP_000151784.1 hypothetical protein -
HHJ31_RS24785 35138..36271 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
HHJ31_RS24790 36438..37211 - 774 WP_000905949.1 hypothetical protein -
HHJ31_RS24795 37224..37724 - 501 WP_000528931.1 hypothetical protein -
HHJ31_RS24800 37989..38219 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HHJ31_RS24805 38216..38632 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sitABCD iucA / iucB / iucC / iucD / iutA 1..65217 65217


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T155039 WP_001159868.1 NZ_CP051721:c33805-33500 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T155039 NZ_CP068610:870763-871173 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT155039 WP_000813634.1 NZ_CP051721:c34025-33807 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT155039 NZ_CP068610:870516-870782 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References