Detailed information of TA system TA155033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3768291..3768649
• Replicon: chromosome
• Accession: NZ_CP051719
• Organism: Escherichia coli strain SCU-116

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: HHJ31_RS18115
• Protein ID: WP_001332330.1
• Coordinates: 3768527..3768649 (+)
• Length: 41 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: HHJ31_RS18110
• Protein ID: WP_000125566.1
• Coordinates: 3768291..3768524 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ31_RS18085	3763364..3763651	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
HHJ31_RS18090	3763710..3765041	+	1332	WP_001183207.1	MFS transporter	-
HHJ31_RS18095	3765149..3765679	+	531	WP_000600749.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
HHJ31_RS18100	3765672..3767534	+	1863	WP_000377122.1	glutathione-regulated potassium-efflux system protein KefC	-
HHJ31_RS18105	3767726..3768205	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
HHJ31_RS18110	3768291..3768524	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HHJ31_RS18115	3768527..3768649	+	123	WP_001332330.1	CcdB family protein	Toxin
HHJ31_RS18120	3768690..3768842	+	153	WP_146692624.1	CcdB family protein	-
HHJ31_RS18125	3768839..3769687	-	849	WP_000257201.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
HHJ31_RS18130	3769694..3770071	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
HHJ31_RS18135	3770074..3770895	-	822	WP_001065363.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
HHJ31_RS18140	3770892..3771881	-	990	WP_000241229.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
HHJ31_RS18145	3771881..3773167	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-39)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4563.34 Da
• Isoelectric Point: 10.9266

>T155033 WP_001332330.1 NZ_CP051719:3768527-3768649 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPI

Nucleotide

• Length: 123 bp

>T155033 NZ_CP068607:c9300-9025 [Klebsiella pneumoniae]
ATGACCACAAGGACGATTGAATACTCAGGCCAGTTCCAGAAAGATGTGAAGAAGGCTCAGAAACGTCATAAAGACATGAG
CAAGCTTAAAGCCCTTATGACGCTGTTAATCAATGATGCCTTGCCATTGCCTCAAGTCTACAAAGATCACCCTCTACAGG
GGAACTACAAAGGCTATAGGGATGCTCACATAGAACCGGACTGGCTACTCATCTACAAGATAACTGATGAAGTCCTACGC
TTTGAGCGAACCGGAACACATTCAGACCTGTTTTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT155033 WP_000125566.1 NZ_CP051719:3768291-3768524 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT155033 NZ_CP068607:c9564-9304 [Klebsiella pneumoniae]
ATGGCCGCAAATGCATTTGTTCGTGCTCGTATAGATGAAACACTGAAAAATGAAGCTGCAGTAGTGCTTGCCGGAATGGG
CTTAACCGTTTCCGATCTGGTTCGTATCACTCTGACTAAGGTAGCCAAAGAAAAGGCTCTGCCGTTCGATATGCGTGTTC
CTAATGAGCTTACTGCTAATACCATCGCTAACAGTGAGAAAGGCGTAGACGTTCATACAGCTAAAGATGCTGATGATCTG
TTTAATCAGCTTGGTATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9